This explanation can be demonstrated in table 3 also because the results of the reactions demonstrates that chloride reacted more by the color of the product compared to the color of chloride in the mineral oil. Also it also demonstrates that iodine is the weakest oxidizing agent because the results show that iodide didn’t change much from the color that it showed in the mineral oil. In example 6 we have shown the activity series for the halogens used in this
Results and Discussion For the first part of the experiment (Part A), five different 100 mL volumetric flasks were each filled with 1,2,3,4 and 5 mL of iron (II) solution. Then 5 mL of YY ligand, were poured to each of the five flasks. Each flask had 5 mL of 2M sodium acetate and 4 mL of 3M NH2OH. Then the whole solution was diluted up to the 100 mL fill mark with distilled water. This was the solution that was used in order to obtain the absorption spectrum for each of the different iron (II) ligand examples different flasks.
After that, dissolve the sample in 2 mL of deionized water and shake the test tube for 1 to 1 ½ minutes to dissolve the solid. Place another dry test tube in a 50mL beaker and weigh it. Find a bottle of barium iodide and record the name and molar mass. Then, weight out either anhydrous barium iodide or barium iodide dehydrate into this test tube and dissolve is it in 2 mL of deionized water. Pour the contents of one of the test tubes into the other and a reaction should occur and you should see a white precipitate of barium sulfate form.
Added one dropper full of Iodine to S+, S-, and tube #2, and agitated the tubes. 7. Added 2 mL of Biuret’s Reagent to P+, P-, and tube #3, gently agitated the tubes and allowed to sit for two minutes. Results: Macromolecules Present in Milk | Test For | Coloration of Positive Control | Coloration of Negative Control | Milk (+ or -) | Red. Sugars | Reddish Brown | Blue | + | Starch | Bluish Black | Brownish | - | Proteins | Purple | Blue | + | Figure 1.
Materials and Methods Part 1 For the cation elimination test first 10 drops of potassium, iron (III), zinc (II), copper (II), and cobalt (II) were added to 5 centrifuge tubes and the color was recorded. Then for the metal hydroxide test, 6 M NaOH was added drop wise till a precipitate was formed. Each solution except potassium formed a precipitate, so then 10 additional drops of NaOH were added to the remaining solutions. Tubes were cleaned with distilled water and 6 M HCL. Next was the ammonia test 10 drops of each metal solution were added to new centrifuge tubes and 15 M NH4OH was added until the solution changed color or a precipitate was formed.
We now slowly pour the solution into a funnel with filter paper. The extract along with 2 pieces of Iodine are added to a new beaker and left for 10 minutes. Finally to determine if the lab was successful, three tests are conducted for Iodine, Iodide and triiodide. The objective is to produce a tincture of iodine by extracting iodide and other components from seaweed. Warm up Activity 1.
The known nutrient solutions were used to create a base-line for protein, starch, and sugars. As listed in Table 1; protein (5g/L), Starch (0.2g/L), and sugar (20g/L) were separated in to 9 different test tubes at 2ml a piece, 3 per nutrient solution and tested for colormetry with the 400ug of the three reagents Lugol’s, Biuret, and Benedicts. Further steps were taken with the nutrients treated with Benedict’s reagent and they were heated in a water bath until they reached a constant 65 degrees C for 7 minutes and let cool to see color change. Distinguishing Organic Molecules in Unknown Dietary Supplements The same reagents used in setting the baseline were used to test the unknowns for nutrient content. Each of the 3 unknowns was distributed by dispensing 2ml of sample solution in to three test tubes.
4. The graph paper is then cut to size and then dipped into the beaker to wash off the egg cysts into the water, it is left for approximately 3 minutes and then the graph paper is removed using forceps. 5. Beakers A, B, C, D and E are placed into five respective incubators at temperatures 5 ̊C, 10 ̊C, 20 ̊C, 30
Obtain a clean-dry test tube. Place 0.3g of the unknown substance in the test tube. Next, add 10mL of distilled water to the test tube. Mix with a stirring rod until unknown is dissolved. 2.
This solution was then cooled in an ice-water bath while frequently being stirred for 10 minutes. Once cooled, 0.25 mL of concentrated hydrochloric acid was added to the solution. The diazonium salt of the anthranilic acid separated. This solution was kept in an ice-bath until further use. The next step of the lab was to prepare the methyl orange by coupling the diazotized sulfanilic acid, sulfanyl diazonium chloride