Lab 5: Osmosis Purpose The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities. Hypothesis Since the 1% sugar solution is being placed into the same type of environment (1% sugar solution), the result should be no change in weight of the simulated cell. However, when the 1% sugar solution is placed into a 50% sugar solution environment, the cell will shrink in size because of the hypertonic tonicity; the water will rush out of the cell to create equilibrium through osmosis, resulting in the shrinkage of the cell. On the contrary, when a 50% and a 20% sugar solution are placed onto an environment of 1% sugar solution, respectively, the cells will bloat up and explode (possibly not in this experiment depending on the strength of our tubing) due to the hypotonic tonicity; the water from the 1% sugar solution will rush into the cell to create an equal amount of solute-to-solution ratio inside, and outside, of the cell. Materials & Methods Materials: · Scale · 4 6” Dialysis Tubing · 4 Transfer Pipets · Sugar · Scissors · Rubber Bands · 4 Same-Sized Coffee Cups · 250ml Graduated Cylinder · Tape Measure · Sauce Pan · 3 600ml Containers · Plastic Covering · Spoon Methods: 1.)
Do an initial Benedict's test on the 15% glucose/1% starch and the beaker solutions for glucose by putting some of the solution and a roughly equal amount of blue Benedict's solution in a test tube, placing the test tube in boiling water for 90 seconds, and observing whether or not the solution changes color from blue. 3. Form a bag out of dialysis tubing by tying off one end, putting in enough 15% glucose/1% starch solution to fill it halfway, and tying off the other end leaving the other half of the bag void of anything (even air). Write down the solution's color. 4.
Then by filtering, iodide can be extracted which when combined with other substances can make an iodine tincture, that is a pharmaceutical product. * Can a single substance be isolated from a mixture? Yes a single substance can be isolated from the seaweed mixture by using methods such as filtration and evaporation. In our experiment we used filtration using a filter paper funnel to extract the iodide from the tincture. * What is this substance This substance is iodide Confidence Report The aim of extraction, is to separate a part of raw material, using a solvent such as water with heat.
MYTH! Drinking water will only absorb into your body which means water will only hydrate you. - Drinking lots of water leads to weightless. MYTH! It doesn't, it only leads to losing water weight.
Akella Turnbull Date: February 2, 2015 Lab Partner: Shubh Rana South Street Seaweed Seaport Lab Summary: In this lab, we were able to make our own tinctures of iodine by extracting iodine from a Japanese seaweed, referred to as Fucus seaweed. We were able to conduct a hot water extraction from this seaweed in order to isolate the iodide, which is a solution that can be used to manufacture a pharmaceutical product. In order to prepare a tincture of iodine for testing we had to use a balance, ignite a Bunsen burner, and heat and filter a solution. In doing so, we had to prepare 6 grams of dry seaweed by cutting it into ½ inch pieces and placing it into a beaker filled with ¾ distilled water. Then after agitating the seaweed to remove monosodium glutamate and pouring off the water, 40 mL of distilled water was added to the seaweed and heated.
After that, dissolve the sample in 2 mL of deionized water and shake the test tube for 1 to 1 ½ minutes to dissolve the solid. Place another dry test tube in a 50mL beaker and weigh it. Find a bottle of barium iodide and record the name and molar mass. Then, weight out either anhydrous barium iodide or barium iodide dehydrate into this test tube and dissolve is it in 2 mL of deionized water. Pour the contents of one of the test tubes into the other and a reaction should occur and you should see a white precipitate of barium sulfate form.
Vinegar, Antacid, and Soap all became positive solutions after I put in the enzyme. A change in pH disrupts an enzyme's shape and structure. pH measure acidity--water is neutral and has a pH of 7. When the pH changes an enzyme's structure, the enzyme can't do its job. Changes in pH break the delicate bonds that maintain an enzyme's shape.
Transfer the distillate via Pasteur pipet into a 15-mL centrifuge tube. Wash the distillate with two 2.5-mL portions of water, removing and setting aside the water layer at each step. Transfer the organic material to a small, dry Erlenmeyer flask, leaving behind the last drops of the water layer. Remove any drops of water that were accidentally transferred to the Erlenmeyer flask, if any, then add small amounts of anhydrous sodium sulfate as drying reagent. When the liquid is dry, it will be transparent and some of the drying agent will flow freely like beach sand.
We pour a drop or two of water onto one piece of paper bag and another drop of oil onto a different piece of paper bag. After a couple of minutes we identify for lipids. If the paper bag leaves an oily spot then lipids is active. Expected Results- The hypothesis of all experiments were true. Protein is in fact active when the liquid turns purple due to biuret reagent.
In this lab, two different titrations were performed with three different antacids to determine which brand is the most effective at the cheapest price. The antacids were ground up separately and approximately 0.2 grams of it was placed in a flask. Methyl Orange, an indicator, and a stir bar were added into the flask. The flask was then put on a stir plate which was under a buret with 0.1M hydrochloric acid. The acid was poured into the flask until there was a permanent pink colour.