DNA Fingerprinting: Revealing the Truth Name: Zainab Khatoon Due Date: March 19th, 2012 BSC2010L.019S12 Course Name: Biology I Cellular Processes Lab Section: 019 Lab Partner: Hiba Fatima Materials and Methods Restriction Enzyme Digestion Procedure: As with all experiments done in this lab, preparation was accounted for with gloves and goggles. To begin the restriction enzyme digestion procedure, microtest tubes were labeled 1 through 4 for different reactions of restriction enzyme digestion. Then, with the use of the P20 micropipette, ten microliters of Enzyme Reaction Buffer were dispensed into each of the reaction tubes. After the Buffer was dispensed, a fresh micropipette tip replaced the old one. The tip of the micropipette
Burst the cellular membrane and the nuclei What is the name of the process used to amplify DNA? Polymarse Chain Reaction How is the amplified DNA sorted? By size What # did the DNA profile match? #3 Why was Greg’s DNA profile in CODIS? Because all forensic scientists are required to have their DNA in CODIS Toxicology Lab: Where is vitreous humor normally located?
P5 First I collected four test tubes; in each test tube I purred the solution that has been labelled A, B, C, and D in the container. Then I added a few drops of Iodine solution to the four test tubes. Container A was starch, because the colour changed to blue black, which is test for starch. Starch iodine blue black I purred the three solutions into the sink. Then I purred the solution from the container B, C, and D in to the three
Enzymes are specific; they only work with certain substrates. Substrates are the reactants in an enzymatic reaction. Out group conducted two labs which examine how specific an enzyme is to a substrate and how much environmental factors affect enzyme reactions. Lactase is the enzyme that breaks down lactose into galactose and glucose. Since they’re both a six sided sugar, lactase can only break down lactose.
Lipids- The materials used in this section were Water, piece of brown paper bag, and vegetable oil. Procedures- Protein-In this experiment we are testing for protein in each solution. We pour drops of biuret reagent into the different contents. If the liquid turns purple, then protein is active. Carbohydrates- In this experiment We test for starch.
An Investigation into One’s Genotypic Frequency of the D1S8o Allele by way of the Polymerase Chain Reaction and Polyacrylamide Gel Electrophoresis Analysis (Alex Devlin, May 17, 2011 Kitchener Waterloo Coligate And Volcational School) Abstract: This lab was done to determine the frequency of our D1S80 allele. The methods that were taken to perform this experiment were DNA isolation, Polymerase Chain Reaction (PCR), and Gel Electrophoresis. In the DNA isolation we used Chelex and Proteinase K to strip the DNA. A thermal cycler, vortex and centrifuge were used to help remove the metal ions and keep the DNA sample mixed. During PCR the DNA was amplified using a primer and reaction mix, multiplying the amount of DNA in the sample.
Genetic Transformation of Escherichia coli with pGLO Ahmed Islam Abstract Aim: This experiment is designed to help understand the concept of genetic transformation. This is the uptake of DNA fragments from the environment by a competent bacterium. Competency must be induced in bacterium such as Escheria coli. Also, this lab helps understand the concepts of plasmids, specifically pGLO, and their genes, specifically green fluorescent gene (GFP). Expression will be regulated using promoters.
Also the position of the bands tells you the comparison between the weights of the molecules within the sample. The stacking gel is needed to pack all the proteins in one band, so that they will start migrating in the running gel all at the same time. In the stacking gel the solutions added were acrylamide, 1 M Tris HCl pH 6.8, SDS, distilled water, APS, and TEMED. The resolving gel allows separating the proteins in the samples based on their weights. Analyze the bands in the gel: 1) 1 band- 1 subunit (monomer)- Integrase - degraded protein under the band with low intensity 2) 1 thick band- two identical subunits- beta complex- ran as a single band, the more concentration the thicker the band is going to look.
The third bag had 2mL of solute and 6 mL of water. And finally, the control bag which was the fourth was 8mL of distilled water. Our independent variables for this portion of the lab was the solute concentration, since we would be changing that for each dialysis bag, while the dependent variable was the change in weight. This allowed us to determine the rate of osmosis based on the solute concentration differences. We kept as many variables as possible the same such as; the water bags type, the amount of time left under water, the water temperature and the way we weighed the bags.
INTRODUCTION: I am trying to investigate enzyme concentration and the rate of reaction. In this investigation, a proteolytic enzyme is used to catalyze the breakdown of protein into peptides. Protein gelatin is the substrate used. As the reaction progress, the gelatin is broken down and become less viscous. This has effect on the time it takes for the gelatin to go through the funnel in to the beaker.