Chem 131A: pH and Buffers Experiment This experiment is designed to review your background in pH and buffers and to expand upon it. The first section involves the titration of an unknown amino acid through its two equivalence points. The equivalence point data will allow you to determine apparent pK values which you will correct for ionic strength and the molar mass of your unknown. Further, you will examine how the amounts of the major species change along the titration. The second section examines the effect of ionic strength on the pK of a weak acid.
Procedure: Before beginning this lab please take the time to review this hand out and compare it to the information you know about organic molecules. Then, develop a hypothesis using the If, and Then statement. Go to http://www.okc.cc.ok.us/biologylabs/ and click on the “Organic Molecules” link. Read the introduction, and then proceed with tests for carbohydrates, proteins, fats, and foods. Record and answer all questions AS YOU GO.
MAINTAINING A BALANCE 1.1 Identify the role of enzymes in metabolism, describe their chemical composition and use a simple model to describe their specificity on substrates. - Enzymes are a biological catalyst found in living things that helps control which chemical reactions are to take place. - Most enzymes are made of protein. [pic] 1.2 Identify the pH as a way of describing the acidity of a substance. - pH is a way of describing the acidity or alkalinity of a substance.
SHORT ANSWER (Enter your answer in between each question (2-8 lines should suffice—depending on the question) 1. Suppose a drug had the same size and shape as a substrate and fit into an enzyme’s active site. Explain how the drug could affect the enzymatic reaction rate. What is the term for this type of substance or reaction? (2 marks) A drug of same size and shape, as that of a substrate, which fits into the active site of an enzyme will act as a competitive inhibitor and the reaction is termed as competitive inhibition.
The experiment will be useful in identifying various proteins (enzymes, hormones, antibodies, etc.) which might be the source or the cure for a certain disease. 11) What is the molarity of 1% of Glycine (Mol. Wt. of Glycine is 75g/mol)?
Importance of biochemistry can be seen from the fact, that it is used in many aspects of daily life, like in clinical diagnosis, manufactured of various biological products, treatment of diseases in, nutrition, agricultural. Biochemistry helps one to understand the biochemical changes and related physiological alteration in the body. * List a balanced chemical equation for each of these chemical reaction types and explain how it is used in your example above As a simple example of how to balance an equation, let us take the reaction which occurs when a large excess of mercury combines with bromine. In this case the product is a white solid which does not melt but instead changes to a gas when heated above 345°C. It is insoluble in water.
Quantitative data deals primarily with numerical characteristics, such as the weight or amount of a particular substance. Qualitative data, however, refers to observable characteristics such as taste, touch, smell, etc. It should be noted, however, that qualitative data can be converted into quantitative data, provided that there is a numerical rubric or basis for gradation. In chemistry, qualitative data allows chemists to generally identify the presence of certain types of ions, elements, compounds, etc. that are present in a sample, by generally observing the physical and chemical properties of the sample when they react with compounds with known reagents.
By, solving the system of equations using linear algebra, the concentrations of the indicator and the conjugate base were determined. The Henderson-Hasselbach equation predicted that the pH for these solution plotted against the log of the ratio of the two species would yield a line whose Y-intercept equaled the pKa for methyl red. The value obtained was 4.96(1), which has a percent error of 1.9. Perhaps, if this were corrected for temperature there would be even greater agreement with the accepted value. INTRODUCTION The purpose of this experiment was to determine the pKa of an acid-base indicator (methyl red).
Proteins are extracted from animals and humans as a mixture in a serum of body fluids. When immunologists want to study a specific protein, like an antibody, hormone, or enzyme, they need to separate it from the mixture. The technique is used for the separation of sugar, Polypeptides, Proteins, Polystyrenes, Silicones, Liquids, Butyl rubbers and polyethylenes. Types: MEC is a one of several types of HPLC. There are basically two types of MEC.
Gel Filtration of Proteins Session 4 Lab Report Semester 2 Victoria Franks 2930499 Gel Filtration of Proteins Introduction The aim of this experiment was to separate a range of proteins from a single sample, using their differing sizes to tell which protein was which when passed through a gel barrier. This method is called size exclusion chromatography. A range of coloured proteins and dyes are used so that samples can be easily idenitifed by the naked eye; however, two of the proteins are a similar colour, making it necessary to use spectrophotometry to tell the difference between them, based on the fact that they have different levels of absorbancy. A gel called Sephadex is used within a tight column and the protein sample is passed through using a strong buffer to help the smaller molecules of the protein sample to diffuse into the gel. As the Sephadex forms a network with tiny holes, this prevents molecules that are too large from getting inside.