There is a space between the peptidoglycan and the secondary cell membrane called the periplasmic space (Betsy, T., & Keogh, J. 2005). Initially Gram staining begins with the basic crystal violet dye and both Gram-positive and Gram-negative appear purple. Then the bacteria are treated with Gram’s iodine, a mordant used in gram staining to intensify a stain (Betsy, T., & Keogh, J. 2005).
Radu Purtuc | Chemistry 231 - Lab | Section 32 Ronnie :: team-member TECH 705, SEPARATING ACIDS AND NEUTRAL COMPOUNDS BY SOLVENT EXTRACTION - MACROSCALE EXTRACTION July 6, 2006 Lab Report INTRODUCTION Purpose: using extraction technique to separate acetanilide, p-toluic acid, and p-tert-butylphenol; then measure the melting points of each compound and comparing them to the statistical values. Background: separating acids and neutral compounds by solvent extraction is a useful technique used in practice by chemists to separate organic compounds from mixture of other compounds. It generally involve several steps, with limiting factors being the number and output purity of compounds to be extracted from source mixture. A number of specific compound solvents not inter-miscible are used to separate and dissolve compounds, and a physical transfer between solvents is employed. An example of use is cleaning compounds of impurities, where different solvents work as a molecular filter that interacts differentially with compound and impurities respectively.
Use a protractor to measure the angle from the reference line to the lines drawn in step 6. 8. Record the angle created by the laser pointer through the air as the Angle of Instance and the angle created by the laser pointer through the water as the Angle of Refraction. 9. Use Snell's Law to determine the Index of Refraction of Water.
However, when the dead skin from the epidermis occasionally blocks a sebaceous gland of a hair follicle, the bacteria may infect surrounding tissue resulting in inflammation and causing a pimple. 4. a) What is the pH of a substance? • In chemistry, pH (Potential Hydrogen) is a measure of the acidity of an aqueous solution. b) How does the pH describe the acidity of a substance? • It uses a scale to describe the acidity level and the pH scale ranges from 0 to 14.
According to the Bronsted- Lowry Model, aids donate an H+ ion to another substance, while bases can accept an H+ ion. On the other hand, the Arrehenius Model states that acids produce H+ ions and bases produce OH ions when submerged in water. The hypotheses made states that all of the household substances are acidic for the following reasons: vinegar, due to its common use as a cleaning agent, to break down grime, as well as detergent, for its use in removing stains from clothing. Soda was determined to be acidic, because it destroys the enamel on teeth, along with ammonia, a powerful cleaning agent known for breaking down dirt. Drain cleaner unclogs drains by breaking down the substances caught in the drain due to its acidic quality.
* What is this substance This substance is iodide Confidence Report The aim of extraction, is to separate a part of raw material, using a solvent such as water with heat. A tincture of iodine is a common medicine sold in many pharmacies, used as an antiseptic on cuts of the skin. The objective of the seaweed lab, is to produce a tincture of iodine by extracting Iodide and other components (seen when a mixture of iodine is present), from seaweed. We
Nuclear envelope What genetic material is found in this part of the cell more than anywhere else in the cell? RNA C. Nucleolus D. Cytoplasm E. Mitochondria F. Lysosomes G. Endoplasmic Reticulum H. Golgi apparatus I. This part of the cell is reponsible for breaking down organic molecules. J. Ribosomes K. centrioles L. Chromatin What function do peroxisomes serve? Peroxisomes contain a variety of enzymes, which primarily function together to rid the cell of toxic substances, and in particular, hydrogen peroxide (a common byproduct of cellular metabolism).
Subject: Separation of unknown organics by flash column chromatography Introduction Column chromatography is a process that is used as a purification technique. The purpose of this lab is to isolate desired compounds from a mixture. The goal of this experiment is to apply column chromatography to separate two compounds and analyze their purity. It will be accomplished by running the mixture to be analyzed through the top of the column tube and then find purities by a TLC plate after the mixture has been separated. Reactions: Results: Calculations: Discussion: The TLC plate had 5 circles, indicating the 5 compounds, when it was under the UV lamp.
2. What is the purpose of fixing a slide that is to be stained? The fixing slide kills cells quickly by denaturing enzymes, adhere cells to slide better to prevent wash off. 3. Why are the specimens to be stained suspended in sterile saline or distilled water?
Chlorophyll (like heme in red blood cells) is an example of porphyrin. In this experiment, chlorophyll and ß-carotene were isolated using the technique of column chromatography. Column chromatography is a method used to separate and purify components in a mixture. In gravity column chromatography (used in this