Effect of Enzyme Activity on Temperature Aim: The aim of this experiment is to investigate how effectively the enzyme amylase breaks down starch at different temperatures Research Question: How does the increase in temperature affect the time taken for amylase to breakdown 2ml of starch? Purpose: Background Information: Enzymes are biological catalysts that comprise the largest and most highly specialized class of protein molecules. Enzymes act as catalysts to increase the rates of chemical reactions. A fundamental property of enzymes is their specificity. Various enzymes have unique shape and chemical composition that creates a site, called and active site.
In an enzyme-catalyst reaction, the substrate binds to the active site and forms an enzyme-substrate complete with the enzyme. (Heller, H. Craig., Gordon H. Orians, William K. Purves, and David Sadava).The enzyme then breaks the bonds in the structure of the substrate. The products of the reaction then leave the enzyme which remains unchanged after the reaction. The enzyme of this experiment was the yeast Peroxidase and the substrate of the experiment was hydrogen peroxide which was diluted by water.
Enzymes are proteins that are used to speed up these reactions without being consumed by them. The activity of these enzymes can be altered by changing their environments, such as enzyme specificity (speed only a reaction that contains their substrate), increasing and decreasing temperature, concentration level, or adjusting the pH level. Catalase is a catalyst that digests potent hydrogen peroxide and converts it into H2O and O. It is due to this hydrogen peroxide digesting ability that we used catalase in this experiment. To record the role that environment plays in the reaction of an enzyme, we exposed the enzyme to various changes in temperature, concentration, and pH.
Introduction This report discusses an experiment to asses the chemical breakdown of starch into maltose (sugar) in the presence of the enzyme amylase, a digestive process within the body. Nutrients can be absorbed only when broken down to their monomers (small molecules that breakdown further to other molecules). Enzymes are large protein molecules produced by body cells. “They are biological catalysts, meaning they increase the role of a chemical reaction without themselves becoming part of the product. Digestive enzymes are hydrolytic enzymes.
5. Describe how temperature and pH affect sucrase activity. Introduction Enzymes are usually protein molecules that act as biological catalysts. A catalyst greatly increases the speed of a chemical reaction by lowering the activation energy necessary to get the reaction started without itself being altered or consumed. On the surface of the enzyme is an active site that temporarily binds the reactants or substrates forming an enzyme-substrate complex.
Investigation of the effect of Substrate concentration on Catalase activity Research Question: To investigate enzyme kinetics, using catalase enzyme from the yeast extract. Background Information: Enzymes are proteins which catalyze reactions that take place in the body or they increase the rate of the biological reactions. In an enzyme catalyzed reaction, the substrate binds to the active site and forms the enzyme-substrate complex with the enzyme. The enzyme breaks the bonds present in the substrate; the final product of this reaction leaves the enzyme which remains unchanged after the reaction. Catalase is a substance which is produced by the liver to break down hydrogen peroxide.
JUN 24 2011 Aim To find out at what temperature does catalyse enzyme work best in and in what optimum temperature the rate of reaction increases. Theory Enzyme is a substance of protein that helps digest food all around our body. Enzymes have the ability to catalyse reactions in living organisms. Catalyse enzymes are found all around our body especially in our liver. Catalyse enzyme creates hydrogen peroxide in to oxygen and water.
Once these two components come together, certain chemical bonds within the substrate molecule change much as a lock is released, and just like the key in this illustration, the enzyme is free to execute its duty once again. Many chemical reactions do proceed but at such a slow rate that their progress would seem to be imperceptible at normally encountered environmental temperature. Consider for example, the oxidation of glucose or other sugars to useable energy by animals and plants. For a living organism to derive heat and other energy from sugar, the sugar must be oxidized (combined with oxygen) or metabolically "burned" However, in a living system, the oxidation of sugar must meet an additional condition; that oxidation of sugar must proceed essentially at normal body temperature. Obviously, sugar surrounded by sufficient oxygen would not oxidize very rapidly at this temperature.
The products of this stage are passed down into the next stages. The 2 molecules of pyruvate are passed down to the oxidation of pyruvate, and NADH will be used for the electron transport chain. The rest of the products, 4 ATP, ADP, and P, are used where needed in the cell. After glycolysis occurs, oxidation of pyruvate takes places in the mitochondrial matrix. During this stage,
Proteins they make up most of the cell structure including the cell membrane. Genetic information in the cell is stored as Protein in the form of DNA. All the enzymes, that catalyze metabolic reactions in the human body, are protein in nature. The recommended amount a person should consume is 0.8 grams per kilogram a person weights (0.36 per pound). Major sources of protein are red meat, fish, eggs, beans, and dairy.