Then by filtering, iodide can be extracted which when combined with other substances can make an iodine tincture, that is a pharmaceutical product. * Can a single substance be isolated from a mixture? Yes a single substance can be isolated from the seaweed mixture by using methods such as filtration and evaporation. In our experiment we used filtration using a filter paper funnel to extract the iodide from the tincture. * What is this substance This substance is iodide Confidence Report The aim of extraction, is to separate a part of raw material, using a solvent such as water with heat.
Magic marker inks are often mixtures of several compounds. Paper chromatography is a common method of separating various components of a mixture. After separation, you can observe the different colors that make up a particular color of magic marker ink. You can also calculate a ratio Rf, which compares how far each compound traveled to how far each solvent (substance that dissolves another substance) traveled during the experiment. Rf = Ds/Df Ds = Distance traveled by the compound Df = Distance traveled by the solvent Materials: coffee filter, tape, isopropyl alcohol (rubbing alcohol), water, 3 different color magic markers (not permanent); 3 identical tall, narrow drinking glasses; metric ruler, 3 pencils Pre-lab Questions: List one extensive and one intensive property of marker ink.
Paper chromatography can be used in separating amino acids and anions, RNA fingerprinting, and testing histamines and antibiotics. (Infromation received from sonic.org Purpose The purpose of this experiment was to separate the dyes that these markers are composed of and show how chromatography works. Materials * Four different markers (including one black permanent marker) * Rubbing alcohol or isopropyl alcohol * Coffee filters (2) * Tall glasses or plastic cups (2) * Pencil * Ruler * Tape * Table salt * Water * Measuring cups/spoons * Clean pitcher or 2-liter bottle Procedure 1. Your first task is to cut the coffee filter into a rectangle measuring three cm by nine cm. You will need 2 for this lab.
4. Use the markers to draw a different colored dot on each of the appropriate pencil marks on the paper strip. Allow the ink to dry, and then go back and add more color to each dot. Before you complete the rest of the lab, make a prediction of the pigment components you expect to see from each marker’s ink. 5.
Purpose- The purpose of this lab was to help explain how the eye sees different wavelengths as colors. This is congruent to what we’re being taught because it helps further the understanding of wavelengths (including frequency, spectrum, emission etc.) also it shows the effect other chemicals have in a chemical reactions. Procedure- -Fill a 250-mL beaker halfway with distilled water. -Get six wooden splints that have been soaked in water.
Plant Pigments Depending on Photosynthesis. Is the rate of photosynthesis faster when there are extreme light and temperatures present? Paper chromatography is a technique used for separating and identifying pigments. The solvent DPIP, (2, 6-dichlorophenol-indophenol), which is often used to substitute for NADPH, moves up the paper by capillary action. As the solvent moves up the paper, it carries along any substances dissolved in it, which are the pigments.
This was confirmed by the lab manual in page 30 which contains the list of the different Rf values by decreasing value. Introduction: Chemist use many ways of separating and determine want components are in different materials. One of this ways is by dissolving the material in solvent this will only get the mixture of components for example the spinach had to be grind up with hexane to get the organic material separated from the pigments that will be separated even further. Next using a solvent and a polar compound like alumina, the pigments separated from the organic material could be run in a column in which those will be separated even more using polarity. As a more polar solvent is use to push the different rings of pigment, these are collected in their own test tubes to then be run in a TLC which will determine the polarity using the Rf values and then comparing them to the table in the organic lab manual ones.
0.9 0.8 0.7 Absorbance 0.6 0.5 0.4 0.3 0.2 0.1 0 400 450 500 550 600 650 700 750 λ max 500 nm 550 nm • • Make sure you understand the directions for using the spectrophotometer and have them written in your manual. Complete the questions on the prelab page. Introduction: There are many different chemical methods that can be used to determine the concentration of a solution. You have already used one method, titration, to find the concentration of Ca+2 ions in a sample of water, and another, density, in finding the concentration of ethanol. In this lab you will use a method called visible spectroscopy to determine the concentration of a solution of CoCl2.
Carbohydrates- In this experiment We test for starch. We pour drops of Iodine onto the different liquids. If starch is present the liquid is to turn a blue/black color. Lipids- In this experiment we are testing for lipids. We pour a drop or two of water onto one piece of paper bag and another drop of oil onto a different piece of paper bag.
Title: Separating the Components of a Ternary Mixture Abstract: This experiment demonstrates how to separate the components in a ternary mixture via five methods, four physical and one chemical. The four physical methods are gravity filtration, evaporation, decantation and extraction; while the chemical method is by selective reaction. The components of this mixture are SiO2 (s), NaCl (s) and CaCO3 (s). Via filtration with the filtrate water the residues were NaCl(aq), SiO2 (s) and CaCO3 (s). The subsequent evaporation of the of the water from NaCl(aq) resulted in NaCl (s) which was cooled to room temperature and then weighed.