The agar plate with the –pGLO LB / amp add no bacteria living in it because it wasn’t ampicillin resistant. But on the other hand the +pGLO LB/amp had a colonies of bacteria because when there is the pGLO plasmid it makes the e-coli bacteria ampicillin resistant so that is the reason why there are colonies of bacteria on the agar plate. For the last agar plate left the +pGLO LB/amp/ara this agar plate is the one that genetically transformed this is the one that could glow under the UV light <Fig 3 bottom right>. The reason that it glowed is because of the arabinose gene on the agar plate, that arabinose gene is the gene that triggers the GFP gene to express
Name: Brieanne Glanville Date: 1/27/14 Bio 205 Labs 1-2: Exercises 24-25 Exercise 24: Survey of Prokaryotes Question 1 a. Why is it important that decomposers such as bacteria release nutrients? Answer: It is important because they feed on dead organic matter b. What term best describes heterotrophic bacteria that feed on living tissue? Answer: The term that describes heterotrophic bacteria are decomposers Procedure 24.1 Complete these exercises using prepared petri dishes Question2- Observe bacteria next lab session to answer this question.
Discuss why a patient might test indeterminate. Your answer: The patient might test indeterminate if they have not yet seroconvert or it they have not yet produced enough antibodies to yield a positive result 3. How would your results have been affected if your negative control had given an indeterminate result? Your answer: If the negative control is positive this invalidates the results. It could be due to not enough washing in the washing steps to remove nonspecific binding.
Title Section: Ice-Nucleation BIO 115 (Tuesday 10:30) Introduction: Ice nucleation, what is it? Well nucleation is the initial trigger that allows freezing, the formation of ice crystals, to progress. Any compound at the appropriate temperature will change its state from a liquid to a solid. The bacteria added to the solution will act as the nucleus for the formation of the ice crystals given the right conditions. Pseudomonas syringae is a rod shaped gram-negative bacterium with polar flagella.
Hypothesis: If bacteria with + pGLO plasmids that are resistant to the antibiotic ampicillin and have the gene for GFP, colonies with survive and grow on the transformation plates that have LB/ amp. In addition, + pGLO bacteria on a plate with LB/amp/ara will grow and glow green under UV light because of the inclusion of arabinose. In the control plates, -pGLO bacteria that are amp sensitive will not be able to grow on the LB/ amp plates. The other control plates with – pGLO bacteria and no ampicillin added will host a lawn of colonies. IV.
The suspension is then heated at approximately 95®C for 15-20 minutes. It is then cooled at 4®C and centrifuged, a pellet will form, but the supernatant will be used for the PCR procedure because it contains the DNA. (http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.01.01_ANTHRAX.pdf). Most reference laboratories use already commercially prepared, that consist of B. anthracis specific primers and probe mixtures that target the genes that code for the virulent toxin. During the PCR reaction, the forward and reverse primers anneal to the complementary sequence on the DNA and a probe that is labelled with a 5’ dye and a 3’ quencher, anneals to the target gene of the bacterium’s DNA.
Each DNA fragment is inserted into beta-galactosidase on the plasmid. The plasmids also contain the Tetracycline resistant gene which are antibiotics that inhibit the bacterial growth by stopping protein synthesis (Bollon, 1984). Plasmids are transformed into the bacteria. Antibiotic Tetracycline is added to growth medium, Recombinant plasmid replicates and at cell division at least one plasmid segregates to each daughter cell, some bacteria are transfected
My bacterial plates did not grow any bacteria because our sterilization may have been eliminated. 2. LB/A/X+: There should have been a colony of bacteria with several splotches of blue because to new bacteria was created with the pBlu gene. LB/A/X-:There should have been bacterial growth because we put bacteria there and fed it. LB+:There should have been bacterial growth but no blue colonies because there was an anti-biotic acting against it.
In a simple stain a basic dye is added to a population of bacteria that has been fixed to the surface of a microscope slide such that all cells in the population take on the color of the dye used. In the second set of exercises you will be performing a Gram stain (a type of differential stain). Gram staining involves the use of two different basic dyes, one that stains a sub-population of the cells and the other that stains
(1) How it works? PCR is repeated cycling of 3 stages 1-DNA Denaturation The DNA is warmed to 95° C. This will make the hydrogen bonds which are holding the DNA strands together in a helix break easily, which will force the strands to separate and make a single stranded