2. Using the funnel, fill dialysis sacs with approximately 20ml of the specified liquid. Sac #1 and #2 filled with 40% glucose solution, sac#3 – 10% NaCl solution and sac #4 filled with 40% sucrose solution. They have to be weighted (data chart on table 1) and then each of the sac dropped into particular corresponding beaker. Sac #1 placed into beaker #1 with distilled water, sac #2 placed into beaker #2 with 40% glucose solution and so forth.
The extraction process is when a solvent, dichloromethane (15mL) is added to the filtrate in a separatory funnel; the mixture is gently swirled together 3 times, and stopcock is released in between to vent the funnel. Dichloromethane (including the emulsion) is then drained from the bottom into a 50mL Erlenmeyer flask. Same extraction process is repeated on the same filtrate and the dichloromethane is, once again, let out to the same 50mL Erlenmeyer flask as before. The combined dichloromethane solution and water (20mL) is poured into a rinsed separatory funnel. Mixture is gently swirled and drained out into an Erlenmeyer flask.
Introduction: Thyme contains a surprising amount of iron compounds. This experiment enables us to determine the amount of iron(II) present in dried thyme by means of a redox reaction. Method: 1. Weigh accurately about 1g of dried thyme and put into a 250cm3 beaker. Record the mass of thyme used.
Procedure Preparing of Medium Agar Materials and apparatus * Distilled water * Nutrient agar powder * Autoclave machine * Petri dish * Hot plated * Conical flask Procedure 1. 500 ml of agar solution was prepared by using 500 ml distilled water and 10 g of nutrient agar powder. 2. Both distilled water and nutrient agar powder was poured into conical flask. 3.
Procedure 1. Begin to prepare an EDTA solution. Weigh out 3.62-3.64 g of NaH2EDTA and record exact mass. Add the weighed amount to a 250 ml volumetric funnel carefully using a funnel Wash the funnel with water to ensure all of the solid is delivered to the flask Add 100-200 ml of water and mix. Enough water should be added so that the flask is full to the 250 ml mark Tightly wrap the top of the flask with a Parafilm when finished 2.
* The sodium carbonate solution was poured into the beaker with the calcium chloride solution. * A sheet of filter paper was folded in half twice and weighed. (1.1g)/(0.7g) * The filter paper was placed in a funnel in a paper cup. * The contents of the beaker were swirled to dislodge any participate from the sides and then slowly poured through the funnel. * 5ml of distilled water was poured down the sides of the beaker, swirled and poured into the funnel.
By using TLC and IR spectrum, we will see the difference between benzophenone (starting material) and diphenylmethanol (final product). Experimental Procedure: Benzophenone (358mg, 2.0mmol) was dissolved in ethanol (5ml) in a 25ml round bottomed flask. The solid did not completely dissolve, so the flask was swirled with magnetic stirrer bar. In a small test tube, sodium borohydride (87mg, 2.2mmol) was dissolved in cold water (1.5ml). This solution was added drop wise to the stirred ethanolic solution of benzophenone at room temperature.
Gas Solubility: The Fountain Effect 1 A small amount of water is injected into an inverted round-bottomed flask connected by a glass tube to a reservoir of water below it. Soon after the injection, the water from the reservoir rushes into the flask, turning red as it enters and forming a fountain inside the flask. Other color changes are also described [1,2]. MATERIALS FOR PROCEDURE A source of dry ammonia gas (cylinder with valve) 10 mL phenolphthalein indicator solution (To prepare 100 mL of solution, dissolve 0.05 g of phenolphthalein in 50 mL of 95% ethanol, and dilute the solution to 100 mL with distilled water.) 2-liter round-bottomed flask 2-holed rubber stopper to fit 2- liter flask 100-cm length glass tubing, with outside diameter of 8 mm 3-liter round-bottomed flask 2 ring stands 2 rings to support 2-liter flask cork ring to support 3-liter flask dropper 10-cm length rubber tubing to fit over dropper's open end 15-cm length copper wire, 16 gauge gloves, plastic or rubber 90-cm length plastic or rubber tubing solid rubber stopper to fit 2- liter flask pressure bulb PROCEDURE A Preparation Assemble the glassware as illustrated in Figure 1.
PROCEDURE 1. Get the following equipment for your group: burette pipette syringe with piece of rubber tubing attached 1–250 mL beaker 3–100 mL beakers grease pencil stand and burette clamp Each beaker must be clean and dry. Label one 100 mL beaker “ACID”, one “BASE” and one “RINSE”. 2. Pour about 80 mL of NaOH solution into the BASE beaker.
Gravimetric Determination of Sulfate Purpose The purpose of this lab is to determine the percentage of sulfate in the hydrate by precipitating the sulfate as barium sulfate. Materials Filler paper Sodium sulfate Graduated cylinder Bunsen burner Watch glass Beakers (250 mL, 400 mL) Rubber bulb Graduated pipette Beaker tongs Funnel Filter Paper Sodium Sulfate Drying oven Wash bottle Stirring rod Silver nitrate Hydrochloric acid Distilled water Small test tube Procedures First, .4861 grams of sodium sulfate was placed into a clean 400mL beaker. Exactly 200mL of water and 1mL of HCl was added to the same beaker. A watch glass was placed on the beaker and the solution was heated using the Bunsen burner to a gentle boil. The watch glass was removed with the beaker tongs.