4. Using free hand, gently shake the tube of E. coli culture broth to disperse the culture throughout the broth. 5. Grasp the cap of the culture tube with the little finger on the hand holding the inoculating loop and remove it from the tube. 6.
The pipette was then filled with 25.0mL’s of acetic acid and then drained into a conical flask, to speed up the process both of the conical flasks were filled with 25.0mL of the acetic acid. 8. Three drops of phenolphthalein was then added to the flask with the acetic acid. 9. The conical flask was then positioned to just under the burettes dropper which had its stopcock opened to allow a medium drip rate and had the sheet of filter paper slipped under it so that the colour could be seen more earily.
Add 30 ml (2 tablespoons) of clear detergent and mix carefully until the salt is dissolved. This is the extraction solution. * Step 3 – Grate a large carrot or 2 medium sized carrots then add some of the extraction solution or blend the carrot with salt solution, then add the detergent. The consistency should be similar to a thick fruit smoothie. Gently stir the resulting mixture for 30-60 seconds.
b) When pouring agar into the petri dish, pour just enough to fill the dish about half way. c) Although you must work fairly quickly, pour the agar gently to minimize the number of bubbles (bubbles look amazingly similar to colonies when the agar hardens). 4. When you are ready to pour: a) Pull out the container of DMA and remove the cap. b) Open the cover of the petri dish halfway and pour in the agar to just cover the bottom of the dish.
The wick needs to be wet when it is used, and I find that soaking it in a little soapy water helps to break down the water resistance that some of the materials seem to have. You may add the wick when repotting by stringing it through the drainage hole in the pot and up over the top rim, then adding potting mix and the violet. Alternatively you may put the wick in after potting by using a hook (I use a hook made from stiff florist wire, but some prefer a crochet hook, and a long darning needle also works.) Simply push the straight end of the tool into a hole on the bottom of the pot, catch the wicking material in the hook, and pull on the tool at the top of the pot to bring it on through... do watch out so that leaves don't get punctured. The wick should dangle out the bottom of the pot and into a reservoir of water below (without the pot itself making contact with the water.)
Experiment 1: Extraction of Caffeine from Tea The primary objective of the experiment is to separate the caffeine from tea leaves. The mixture of tea and the calcium carbonate is separated from tea leaves by vacuum filtration. The solvent used in extraction of caffeine is dichloromethane. Anhydrous magnesium sulfate is added to the dichloromethane solution to dehydrate the mixture. The dichloromethane is then separated from the mixture in the rotary evaporator.
Then after agitating the seaweed to remove monosodium glutamate and pouring off the water, 40 mL of distilled water was added to the seaweed and heated. During this procedure, the iodine extract was filtered and several tests, both standard procedure and home- Objective: The purpose of this experiment is to understand how a single substance can be isolated from a mixture, like seaweed and water. Also, learn how to utilize filtration and extraction to make a tincture for testing for Iodine, Iodide Ion, and Triiodide Ion. Procedure: 1. 6 grams of dried seaweed, cut into ½ inches should be places into a 150 mL beakers that will later be filled with ¾ distilled or deionized water.
* 100% key lime * 50% key lime * 25% key lime 3. This is the formula to produce different key lime concentrations. * M1V1 = M2V2 Preparing of Kirby-Bauer test Materials and apparatus * Broth cultures of P. anvenginosa, E coli, S. aurens and B. spizizenii * Sterile cotton swab * Forceps * Bunsen burner * Whatman filter paper (small piece after punch) * Key lime discs * Parafilm Procedure 1. Swirl the contents of the broth culture of P. anvenginosa until it is equally murky throughout. 2.
Once it is completely distilled, remove your filtered material and add 2 mL of dichloromethane. Swish the flask, and then place into a small beaker. Next, place the beaker with the distilled liquid on a heating mantle and heat to a gel like substance. Make sure not to burn it. The next processes that will be
6- Place only the edge of the Q-tip at the top the Flame. 7- Remove it when you see the of light being given off to avoid burning the Q-tip. 8- Clean up procedure: Discard used Q-tips to the bin, cover back compounds and put them up in a safe place, pour away distilled water in the sink, disconnect the Bunsen burner and clean it if stained,clean the lab test surroundings with paper towel to ensure no stain is left, wash your hands remove your goggles only when all equipments have been placed in safe places. Compound | Flame Colour Observation | 1 LiNO3