Online Biology Enzyme Lab

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Lab Report: Enzyme Lab Your Name: Samuel Thohammer__________________________________________________________________ Purpose of this Lab What is the goal of this lab? What question is it trying to answer, or what problem is it trying to explain? It is going to explain how a liver's enzymes break down substances that could be harmful.| Hypothesis After reading the lab instructions - but before starting the lab - record your best “educated guess” about what will happen in the experiment. Give your reasons and outline any assumptions that lead you to this hypothesis. I'm guessing that the liver will turn the peroxide into something else, or make it change.| Experimental Design List the materials used in this lab, and the procedure you followed. Commercially available bottle of hydrogen peroxide, from drugstoreLiver, either chicken or beefAn onionA small potatoAn Apple (peeled)Wooden sticks (can be the ones used for shish kebobs)MatchesSharpie marker pen or other pen that can write on a glass surfaceTapwaterSeveral glass kitchen glasses (so you can observe the inside of the glass)Procedure (list steps in the order in which you performed them):Cut two small (1/4 to 1/2 inch long) pieces of liver.Place the liver into two small glasses, labeled “C” and “E”.Pour enough hydrogen peroxide from the bottle to cover the liver in glass “E”, add a little more. Pour the same amount of water into tube “C”. A simple test for the presence of hydrogen gas is to hold a glowing wooden applicator stick at the mouth of the test tube or in the bubbles forming at the surface of the liquid. Hydrogen gas produces a popping sound. A simple test for the presence of oxygen is to hold a glowing applicator stick in one or more of the bubbles inside the tube. If oxygen is present, the glowing end of the stick will flare up and glow brighter. Light a match and burn the

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