Some enzymes lose their activity when frozen. Some enzymes denatured when temperature increases too much, a potentially permanent process. Variables: Independent Variable: Temperature, 0°C, 15°C, 35°C, 45°C, 60°C. This is because it is the only thing we are changing over and over in the lab. Dependent Variable: The time taken in seconds the enzyme to react at different temperatures.
The effectiveness of lipase on temperature Abstract Enzymes are biological catalyst that speeds up the rate of reaction. Different enzymes work best at different temperatures, which is also called an optimum temperature. Different enzymes also have different functions. Lipases are enzymes, needed to break down lipids (fats and oils) into the products - glycerol and fatty acids. They work best at room temperature around 36-40 C. Introduction For this experiment, I will be looking at how the change in temperature affects the rate of reaction.
The reason for this is because; this allows the substrate to bind to the active site, which is known as the ‘lock and key model’. The substrate is the key and active site is the lock. No other key will fit into the lock. There are many factors that affect the rate of enzyme activity in the liver, namely, Ph level, and substrate concentration. I chose to do an experiment on ‘How temperature can affect the rate of enzyme activity in the liver?’ Temperature affects the “speeds of the molecules, the activation energy of the catalytic reaction and the thermal stability of the enzyme and substrate.” (2) At different levels of temperature the affects on the enzyme in the liver varies.
The linear regression model could also be use to approximate the heat of solution. Where finding the heat of solution could be important is when we are trying to figure out the enthalpy change of a solution. Procedure The procedures for this experiment were followed as those printed on pages 221-223 of the manual, experiment number 36 (see references). Deviations in this experiment from the what was printed in the procedure was that Microsoft excel was needed to make the graph instead of using the graph paper provided at the end of the experiment. Data and Results The first eight graphs show the relationship between the temperature of the solution and the nature log of each specific molal concentration.
Title: Environmental Influences Objectives -Determine optimal growth temperature for a bacterium -Learn about the effect of salt concentration on the growth of bacteria. -To determine how the different pH values affect the population growth of bacteria. -To determine how the different temperatures affect the growth of bacteria. -To differentiate between oxygen requirements using anaerobic culture media Introduction Microbes are exposed to a wide variety of environmental factors in addition to nutrients. For most microbes, environmental factors fundamentally affect the function of metabolic enzymes.
Aim: To investigate the effects of changing in PH on the activity of enzyme (trypsin). Hypothesis: Alternative hypothesis: As the PH level goes up, the enzyme activity of trypsin will speed up. The following graph is the prediction supporting my hypothesis. Introduction: Biology background (with theories) What are enzymes? Enzymes act as biological catalysts; they make chemical reactions to work quicker and remains unchanged when the reaction finishes.
However, one must also realize in that high temperatures can cause thermal denaturation, change in the tertiary structure of an enzyme, and freezing may also damage an enzyme as well as heat. (Taggart) PH affects enzyme activity because if the solution is too acidic or too basic the catalase is inactive and no longer will function as an enzyme. Most living things produce enzymes. This enzyme is produced to decompose hydrogen peroxide into water and oxygen gas (Hewitson). Hydrogen peroxide is toxic to living organisms, which is why you put it on wounds to kill germs.
In our 2nd, we examined the effect of warmer temperature. Our research question was, ‘does catalase denature in stages or all at once as temperature increases?’ Our hypothesis was: if temperature increases, then the catalase will denature in stages as the temperature increase, the catalase will slowly stop working. We followed the procedures in the lab manual (choinski and karatit 2014) with the following expetions: For experiment 1, we used 1.5% hydrogen peroxide concentration and experimented this concentration at 4*c, 24*c (room temp. ), 44*c, 52*c and 60*c. For experiment 2, we used 3% hydrogen peroxide concentration and experiment this concentration at 21*c (room temp. ), 35*c, 45*c, 55*c, & 60*c. Figure 1: Effect of temperature on catalase activity.
Hydrogen Peroxide at high concentrations can be lethal. Catalase is there to help with the processes of breaking it down to assure that this doesn’t happen. The purpose of this lab was to help us understand what happens when an enzyme works with a substrate to make the reaction faster. It was to help show us what the effects of pH and temperature on the enzyme’s reaction. My hypothesis for the experiment was that the optimum temperature for catalase to function is 98.6o F because this is the optimum temperature for the human body.
The hypothalamus is the processing centre in the brain that controls body temperature. It does this by triggering changes to effectors, such as sweat glands and muscles controlling body hair. Heat stroke can happen when the body becomes too hot; and hypothermia when the body becomes too cold. Temperature control Temperature control is the process of keeping the body at a constant temperature of 37°C. Sweat is made up mostly of water but also contains minerals, urea, lactic acid, ammonia and sugar.