The components of the sample will be separated on the basis of their ranging physical and chemical properties, imparting different affinities for the two phases. Thin layer chromatography (TLC) was the first chromatographic method for assessing phospholipids, and is commonly used today.
These proteins open and close the membrane. | | 11 CORRECT | | What did Robert Hooke use to study this organism? | | | A) | light microscope | | | B) | electron microscope | | | C) | cork chamber | | | D) | DNA analysis | | | | | | Feedback: Robert Hooke used a primitive light microscope to observe organisms such as this flea, as well as cork, which led him to the notion of cells. | | 12 CORRECT | | What does this depict? | | | A) | cytoskeleton | | | B) | fluid mosaic model | | | C) | impermeable membrane | | | D) | genetic material | | | | | | Feedback: Phospholipids in the plasma membrane create a fluid environment in which other molecules can float, allowing selective permeability.
Your answer: yes;results showed they have partial identity Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Lab Report 1. Describe how the direct and indirect ELISA are different. Your answer: Direct ELISA is directly looking for the foreign substance. The microtiter plate is coated with homologous antibodies made against the antigen of interest If the antigen is present, a sandwich of antibody, antigen and secondary antibody will form Indirect ELISA is designed to detect antibodies that the patient has made against the antigen.The microtiter plate is coated with
Rough ER transports these proteins to the regions in the cell where it is needed. | Ribosomes | A ribosome is composed of two parts known as the large and small subunits. Each of these is a combination of protein and a type of RNA known as rRNA. | the main thing they do is that they arrange the strands of the amino acids for use of the other parts of the cell and ultimately the body. | Goigi Apparatus | The main function of the Golgi apparatus is to be responsible for handling the macromolecules that are required for proper cell functioning | It packages and ships molecules made in the ER.
As a more polar solvent is use to push the different rings of pigment, these are collected in their own test tubes to then be run in a TLC which will determine the polarity using the Rf values and then comparing them to the table in the organic lab manual ones. This is important because we want to know what some things are composed of and by using the polarity in different components makes it easier to determine them. The gain from this experiment is determining the different pigment of spinach and by doing so comparing the polarity of each of them. Then determine why that could be by looking at the structures of the pigments separated. Discussion: The first thing done in
Decant(ing): Decanting is a way of separating a solid from a liquid. We can carefully pour a liquid from a solid, provided the solid is quite dense and resides on the bottom of a container. The easiest way to accomplish this is to hold a glass stir rod against the lip of the container containing the solid and liquid, and gently pour the liquid down the stir rod into a new container. Extraction: Extraction is a way to separate two components with different solubilities, that is, extract one chemical from another. Extraction relies on a principle called serial dilution.
Radu Purtuc | Chemistry 231 - Lab | Section 32 Ronnie :: team-member TECH 705, SEPARATING ACIDS AND NEUTRAL COMPOUNDS BY SOLVENT EXTRACTION - MACROSCALE EXTRACTION July 6, 2006 Lab Report INTRODUCTION Purpose: using extraction technique to separate acetanilide, p-toluic acid, and p-tert-butylphenol; then measure the melting points of each compound and comparing them to the statistical values. Background: separating acids and neutral compounds by solvent extraction is a useful technique used in practice by chemists to separate organic compounds from mixture of other compounds. It generally involve several steps, with limiting factors being the number and output purity of compounds to be extracted from source mixture. A number of specific compound solvents not inter-miscible are used to separate and dissolve compounds, and a physical transfer between solvents is employed. An example of use is cleaning compounds of impurities, where different solvents work as a molecular filter that interacts differentially with compound and impurities respectively.
stores proteins and alot of exporting (sending stuff out of the cell) 3. In which part of the cell would you expect to find nucleotides? building block for DNA, DNA found in the nucleus 4. Name the two organelles involved in energy conversion.chloroplast and mitochondria 5. What are the basic functions of the organelles in chapter 4?
However, different molecules will move at quite different and individual rates depending on the physical characteristics of the molecule and on experimental system used. The velocity of movement, ν, of a charged molecule in an electric field depends on variables described by Eq/ f • Nucleic acid molecules are size separated by the aid of an electric field where negatively charged molecules travel toward anode (positive) pole. The migration flow is resulted solely by the molecular weight where small weight molecules migrate faster than larger ones. In addition to size separation, nucleic acid fractionation using agarose gel electrophoresis can be an initial step for further purification of a band of interest. Extension of the technique includes expunging the desired “band” from a stained gel viewed with a UV transilluminator.
What is the identity of your unknown? Justify your conclusion by comparing the observed molecular weight and pKa values to those for all amino acids that might have been your unknown. B) Examining the effect of ionic strength on the pKa of a weak acid. Restandardize the pH meter with both pH 4 and pH 7 buffers. Why is this necessary?