Mitosis and Meiosis
Lab report by:
Date of lab experiment: 14 July 2011
Part A: Introduction
Deoxyribonucleic acid (DNA) is a molecule that is present in the cells of all living organisms. It is the hereditary “blueprint” of life as it contains all genetic information about the organism in its cells.
The purpose of this experiment is to extract DNA from the cell itself and to be able to see the DNA structure. Chemicals will be used to chemically treated in order to break open the cell and nuclear membranes. The DNA will then be separated from the mixture containing the cell membrane and would then be altered in order for the DNA not to dissolve and able to see it with the naked eye.
Materials used for the experiment are listed down on page 25 of the lab manual.
Method is listed under Procedure on page 25 and page 25 of the lab manual.
* (4) No need in combining cooled mixtures of 2 teams. Instead, every team blends their own mixtures.
* (7) No combination of mixtures and therefore no need to divide onion liquid into 2 portions.
Stringy DNA formed after adding isopropanol to the mixture and spooled out and placed into TE buffer which protect the DNA from degradation and also for storage. ‘
1. Why is detergent used? Why is heat used with the detergent?
Ans: The detergent was used to break down the fat and proteins that make up the cell membrane. In presence of the detergent, the fat and proteins become imprisoned in the soap bubbles and therefore the breaking of the cell membrane.
Heat is to denature the protein so that it is no longer soluble and can easily be removed.
2. Why is the onion mixture processed through the blender?
Ans: When the mixture of the onion and the detergent is processed through the blender, the enzymes in the detergent are breaking down the lipid molecules of the cell and nuclear membrane which releases the contents of the cell including...