Investigating Enzyme Concentrations and Enzyme Activity

995 Words4 Pages
Investigating Enzyme concentrations and enzyme activity Enzymes are substances that are produced by living organisms that act as biological catalysts that speed up rates of reactions for specific chemical reactions. This occurs by attaching a substrate molecule (the substance that the active site of an Enzyme interacts with) into its highly specific active site. In chemical reactions a certain amount of energy is needed to start the reaction, this is called the activation energy. Enzymes lower the amount of activation energy that is needed to make the reaction happen; this in turn causes the rate of reaction to increase. Certain factors affect the rate of reaction:- Temperature – temperature increase the rate of reaction because the molecules gain kinetic energy and this increases the likelihood of an enzyme and a substrate colliding, subsequently increasing the rate of reaction. If this temperature goes over the optimum temperature then the active site of the enzyme will become denatured. The bonds that hold the enzyme together will be broken causing it to lose its 3-D structure, and substrate will no longer be able to fit , so no more E/S complexes will be formed. PH – Like temperature enzymes have an optimum PH. If the PH goes over the optimum PH then the chemical nature of the amino acids can alter. This may cause the bonds that hold the tertiary structure together to break. The active site will be disrupted and the enzyme will be denatured. Substrate concentration - At low substrate concentrations many active sites will not be occupied, decreasing the rate of reaction. At high substrate concentrations most to all of the active sites will become occupied, increasing the rate of reaction. At a certain point increasing substrate concentration will no longer have an effect as most of the active sites will be filled up, so no more E/S complexes will be

More about Investigating Enzyme Concentrations and Enzyme Activity

Open Document