With your fingertips, pinch the sides together of the empty pipette labeled HCl. 13. Gently tip the 24-well plate towards you so that the drops of HCl are puddled together along the bottom rim. Insert the stem of the pipette into the puddle and release the pressure of your fingers to suck all of the HCl into the pipette. 14.
Make sure the rubber stopper is securely in place since gas can easily leak out. Collecting the gaseous product: Prepare the gas collection bottle by positioning the bottle so that its mouth is. directly over the hole at the bottom of the trough; this is where the gas will enter the bottle. After the reaction, slide a square of glass over the mouth of the bottle while the bottle is still under water. Keeping the glass fixed to the mouth, raise the collection bottle and invert.
3. Measure 1/4 c sugar; add to milk/cream mixture in baggie. 4. Close the bag SECURELY, squeezing most of the air out before sealing. 5.
(See figure 1.1.) Figure 1.1 3) Use the masking tape to keep the cone closed. At the closed end of the cone (the bottom), use extra masking tape to cushion the blow from the fall AND weight the bottom. This will insure that the cone falls bottom side first. 4) Next, we will construct a box that is a little larger than the egg with the remaining poster board.
Obtain an Erlenmeyer flask that has a vacuum opening and attach the vacuum tube to it 8. Insert funnel and rinse the funnel with distilled water 9. Turn vacuum on and pour the pink solution into the Erlenmeyer flask, the precipitate should stay on top of the filter 10. Rinse the beaker out until all residue is gone 11. Try to rinse the precipitate in the funnel until the pink color is gone 12.
Slowly pour the beaker contents into the drip paper. Make sure every piece is out of the beaker. 10. Remove the funnel from the drip and the filter paper from the funnel. Allow the filter paper to dry overnight.
Allow the mixture to cool for a few minutes then filter it, using either gravity or vacuum filtration. (We shall be using vacuum filtration.) Wash the residue in the funnel once with a little water and collect all the filtrate. 4. Pour all the filtrate and washings into a 250cm3 volumetric flask.
Use the 1.0mm opening for 1.6mm fiber. Also trim the Kevlar to the ‘C’ length using the electrician style scissors. * Using the marker pen and the template card provided, measure and mark the buffer strip length as shown on the template. * Using the buffer strippers, strip off the buffer in at least two pieces. * Using a dry, lint-free wipe; Remove any remnants of the protective coating on the fiber after stripping the buffer.
Fill the cuvette about 3/4 full of the solution you wish to test. Wipe the outside of the cuvette with a soft tissue (Chemwipe) to remove any moisture or fingerprints from the outside
Care must be taken when squeezing the pipet bulb on the filter pipet. Too much pressure might cause the filter to leak or fall off. Add about 2 mL of fresh tert-butyl methyl ether to the solid in the RB flask, warm briefly, let the solids settle for a minute, and pipet the liquid to the centrifuge tube as before. Again allow the solids to settle briefly in the centrifuge tube, then filter the liquid through the pressure filtration apparatus, into the same 25 mL Erlenmeyer flask. Doing a rinse such as this helps to ensure that any trimyristin that was left behind in the RB flask and centrifuge tube is not lost, thereby helping to ensure that