Hplc Essay

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HPLC Method Development for Vanilla Flavoring Abstract: The concentrations of vanillin, ethyl vanillin, and coumarin into Weis’s Pure Vanilla Extract and Baker’s Imitation Vanilla Flavoring samples were determined using HPLC (High Performance Liquid Chromatography). The concentrations of vanillin in Weis’s Pure Vanilla Extract and Baker’s Imitation Vanilla Flavoring were 1.94 mg/ mL and 7.30 mg/ mL respectively. The concentration of ethyl vanillin in Baker’s Imitation Vanilla Flavoring was 0.690 mg/ mL, whereas ethyl vanillin was not present in Weis’s Pure Vanilla Extract. Coumarin was not present in Weis’s Pure Vanilla Extract and Baker’s Imitation Vanilla Flavoring samples. Introduction: Vanilla is an odoriferous extract prepared from the processed pod of the Vanilla planifolia orchid, and it is used in a variety of ways, but it is especially used for foodstuffs, perfumery, and in aromatherapy.1 This natural vanilla extract mostly contains vanillin. Vanillin can also be made synthetically from low-cost starting materials, such as ethyl vanillin, which has much more flavoring strength than vanillin and is used in formulation of imitation products.2 Some vanilla extracts are adulterated with coumarin, a phytochemical, to increase the vanilla flavor perception since it has a sweet odor and is used as flavoring and fragrance enhancer.3 Coumarin is banned from food products sold in the United States. Yet the Food and Drug Administration (FDA) occasionally has found tonka bean extract products containing coumarin in some ethnic food stores and Mexican restaurants in the United States.4 In this experiment, HPLC (High performance liquid chromatography) method was used to separate the components such as vanillin, ethyl vanillin, and coumarin from their mixture solution. In HPLC chromatography, a mixture will separate by going through a medium in which the
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