We used Polymerase Chain Reaction to amplify samples of the bacterial plasmid DNA. This allowed us to determine whether the three farms have a common form of bacteria that is resistant to tetracycline. In petri dishes each member of the team used dilution of 10-2 to 10-6. With a pipette we put 100μl sample of bacteria. The dishes were placed in an incubator for 24 hours then kept in a cold room until ready for the count of bacteria.
We now slowly pour the solution into a funnel with filter paper. The extract along with 2 pieces of Iodine are added to a new beaker and left for 10 minutes. Finally to determine if the lab was successful, three tests are conducted for Iodine, Iodide and triiodide. The objective is to produce a tincture of iodine by extracting iodide and other components from seaweed. Warm up Activity 1.
September17.2013 P.4 Environmental Sources Of Bacteria Purpose: We did this lab to find out exactly which conditions would help to grow a bacterium the best. Materials: • Agar • Petri dish • Cotton • Incubator • Tape • Bacteria Procedure: 1. Mark bottom of Petri dish 2. Split petri dish into 4 sections and label the sections 3. Pour hot agar into dish 4.
The streak plate technique is done to isolate a colony formed by a single cell from a mixture containing millions of cells. The streak plate technique was also used to obtain pure cultures of the bacteria using a trypticase soy agar (TSA) plate and was incubated at 37 ̊ for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the arrangement of the cells; if an organism appears purple under a microscope then it is said to be Gram positive, and if it
Once the chemicals had been organized I then place 2 drops of the first chemical and 2 drops of the second chemical into the allotted well. Once 2 drops of each chemical were placed in the well I stirred the mixture with a toothpick and observed the mixture. The observations of the various combinations were then noted. The following combinations took place in the experiment. NaHCO3 (sodium bicarbonate - baking soda) and HCl (hydrochloric acid).
Remove towels and re-clean area with disinfectant solution. Decontaminate reusable clean-up items and other reusable equipment. Inform laboratory personnel when the clean-up is complete 6. Describe the procedures that one must do before leaving the lab. (3 pts) Section 2 Biochemical Tests 7.
The visual analysis of the results of the experiment will also be provided in which my qualitative data will be presenting which includes my own evaluation and observations of the experimental results. Quantitative & Qualitative Analysis The tables below show the results obtained by the first experiment in which I tested the effectiveness of TCP on E.coli Bacteria in 2 different petri
Processes such as gram staining can be used in conjunction with monitoring the growth of a bacteria to further reinforce a hypothesis. Materials and Methods: Materials Streptomyces and Antibiosis The following materials and apparatus were used to complete the study of Streptomyces and its antibiotic properties. Soil samples were collected from the pond on the Brooklyn College campus,
Purpose The purpose of the lab was to recognize chemical changes, what they may indicate, and to understand household product chemical properties. The use of common chemicals allows for a better understanding of the properties that are used in every day life and how they affect each other. Seeing the reaction at a macroscopic level allows questions for the microscopic level and further experimentation. Procedure The experiment was started by gathering the proper equipment and supplies listed in the lab manual, while reading through the entire lab experience. The supplies included: a pencil, a printout of the table of reactions, a toothpick, a sheet of white paper, a sheet of black paper, a paper towel, distilled water, a small amount of household cleaners (such as: Windex, vinegar, and stainless steel/granite cleaner), the goggles from LabPaq, 24-well plate, 96-well plate, a empty short stem pipet, and the bag labeled for the experiment from LabPaq that included: aqueous ammonia, bromethylmol blue, Copper (II) Sulfate, FDC blue dye #1, hydrochloric acid, lead (II) nitrate, phenolphthalein solution, potassium iodide, silver nitrate, sodium bicarbonate, sodium hydroxide, sodium hypochlorite, and starch solution 1% stabilized.
The washed polymer will be placed at a filter paper overnight at 60 degrees. b) Change in pH The change in pH level will be measured using the pH meter or indicator during the microbial activity every 10 days for 60 days. c) CO2 Evolution Test Biodegradation of polymer chain leads to production of carbon dioxide which will be measured by Sturm test using a carbon dioxide (CO2) meter. d) Scanning Electron Microscopy (SEM)