The Antibiotic Decrease Yeast Cells Francine Rodriguez Biology 1401 Lab section Department of Biology, The University of Texas-Pan American March 8, 2013 Abstract: The main focus in this experiment was to find out antibiotics affect yeast cell cultures in a controlled environment. We imagined we owned a business in which a part of the business is to grow yeast (Saccharomyces cerevesiae) to be distributed as dry yeast for bakeries and breweries. Profits depend rapid, economical production a large number of yeast cells. We had observed that there is apparent difference in the yield of yeast cell when it comes in contact of an antibiotic. It appeared that that antibiotic had decreased the number of yeast cells.
This is an indicatore because sucrase is an enzyme found in the small intestine that catalyzes the splitting of the disaccharide sucrose into the monosaccharides glucos and fructose. The amount of product produced indicate the level of sucrase activity that occured. 5. Explain why denatured sucrase was used as a control. Denatured sucrase was used as a control because denatured enzymes, in this case sucrase, lose their shape and can potentially lose their ability to catalyze a chemical reaction.
(PubmedHealth, 2009) Fructose 1 phosphate which is the substrate of Aldolase B is split by Aldolase. This splitting is done by the lock and key design because the fructose 1 phosphate fits into the aldolase. The lock and key model enables the molecules called substrates to go through chemical changes to form new substances called products. Substrates are what enzymes act upon. Each substrate fits into an area of the enzyme and is called active sites.
Chemistry PAT Question 1 Polyhydroxybutyrate (PHB) is a biopolymer with properties very similar to that of polypropylene, though has all the benefits that come with biopolymers. PHB was first introduced to the scientific community by the scientist Maurice Lemoigne in 1926. He noticed that to produce PHB you need particular micro-organisms such as Alcaligenes Eutrophus or Bacilius megaterium undergoing physiological stress in an apparatus such as an agar dish to allow the micro-organisms to multiply. In order to achieve this stress there is a restriction to the amount of nitrogen the micro-organisms can attain thereby altering its nutrition. This is quite an efficient method of production and the amount of the PHB polymer yielded is around 30-80% of the dry weight of the micro-organisms used.
The specific substrate in this case that is acted by Aldolase B is fructose-1-phosphate. This is later converted into DHAP and glyceraldehyde. The product, once finished, will then enter the glycolysis cycle to form ATP (Hudon-Miller, 2012c). Role of Aldolase B in the Breakdown of Fructose Aldolase B is found mostly in the liver but can also be present at lower levels in the kidney and intestinal cells. It is involved in breaking down a simple sugar called fructose.
Healthcare applications of Chemical reactions Discussion 1 * What is the difference between biochemical, pharmaceutical, and diagnostic chemical reactions in healthcare? The basic aims of health profession are to get the understanding and maintenance of health and understanding and effective treatment of diseases. Biochemistry greatly effects on both aspects. Biochemical studies clarified many aspects of health and disease, and conversely, the study of various aspects of health and disease has opened up new areas of biochemistry. Diagnostic chemical reactions are those used for medical testing purposes such as a glucose tolerance test.
This specific study can really be useful for future studies since they can try controlling the Type 1 diabetes by modifying the gut microbiota. Also, it can open new doors in trying to come up with new ways to treat or prevent the diabetes. Studies like these are what need to be researched in order to get closer to a solution. Book Reference: Living with Diabetes This book discusses four important main points; Type 1 diabetes, Type 2 diabetes, complications of diabetes, and problem solving for diabetes. It starts off talking about the basics of diabetes and how it’s diagnosed.
Eosin methylene blue and Mannitol agar plates will also be used to determine pH values. Test Three: Gram Stain – A basic technique which will help to distinguish between a Gram-positive and Gram-negative bacterium. Test Four: Catalase assay – Is to be used to determine whether the unknown bacteria will liberate free O₂ gas. Test Five: Oxidase assay – The cytochrome Oxidase will aid in determining whether the unknown bacteria has an absence of Oxidase or not. Test Six: Glucose assay – Whether an organism can respire or ferment glucose can be tested with this Glucose O/F medium assay.
Jameson used data from credible sources such as Centers for Disease Control and Prevention and University of Mainz. The article will be used to show the amount of debt caused by obesity. A story will be included to back up my opinion. A story will also help people to relate to the information. Ogden, C., & Carroll, M. (2010, June 04).
The Pareto chart displays the magnitude of the influence of each factor on the response. The results showed that ammonium sulphate, yeast extract, 1,2 propylene glycol, serine, L-lysine, ornithine, peptone, MgSO4 significantly influence tacrolimus production. Under the present study, R2value (0.9910) indicates the sample variation of 99.1% can be attributed for tacrolimus production to the independent variables and only about 0.9% of total variation could not be explained by the