The two petri dish that contained E. coli and S. Marscensen were placed upside down and allowed the growth of the bacteria to interact with the antibiotics for a week. Results: The bacteria in the petri dish were left to grow for a week and in this process
To determine if hyphae grow at the same rate, graph the results of table 2 and compare the slopes of the 3 hyphae from the slide preparations. 11. To determine if mycalolide B affects fungal tip growth and nuclear position repeat all the steps except in step 2 need to add mycalolide B and let it incubate for 10 minutes. Make sure to label the slides with the
Source: Table 24.4 An evaluation of colony morphology. Colony # | Species | D(mm) | Color | margin | form | elevation | 1 | Bacillus megateriumhttp://dept.kent.edu/microbiology/htm/organism.htm | | Beige/White | smooth | pinpoint | flat | 2 | Rhodospirillum rubrumhttp://commtechlab.msu.edu/sites/dlc-me/zoo/zwp0257.html | | Red | Entire | pinpoint | convex | 3 | Escherichia colihttp://www.britannica.com/blogs/2011/06/evil-coli/ | | Translucent | Undulate | pinpoint | Slightly raised | 4 |
Experiment 11 Lab Assignment Answer Sheet: Chromatography of Food Dyes Name: Pre-Lab: Define the Rf value of a compound The "rf" value is the distance the compound moves relative to the distance the solvent moves. Data and Observations: Record the distance travelled by the sample and the solvent in the table below and calculate the Rf values. Table 1. Color Distance(m Blue1 Blue2 Red3 Red40 Yellow5 Yellow6 33 3 10 22 42 30 .55 .05 .17 .37 .70 .50 m) Rf Solvent 60 Table 2. Kool-Aid®: Grape Kool-Aid®: Strawberry Distance (mm) Red: 5 10 Distance (mm) Blue:17 n/a Rf .08 and .28 .17 Substance Solvent 60 Table 3.
BIOL1003 Methods: Lab 1: Cell Size and Structure THURSDAY Fall 2012 A Leica DME microscope was used for observations of Elodea canadensis, human cheek cell, Paramecium aurelia, Gonium sp., yeast and bacteria. A culture of the aquatic plant Elodea canadensis submerged in water was available in the lab. A single leaf was removed from a stalk and placed in a drop of distilled water on a slide. The preparation was covered with a coverslip and observed in the microscope. The preparation remained illuminated in the microscope for approximately five minutes.
Introduction High performance liquid chromatography (HPLC) is used to separate compounds in a sample, identify compounds and can even be used to deduce the relative amounts of different compounds in a mixture. HPLC works under the same principle as thin layer chromatography using both a stationary and mobile phase. The mobile phase carries the mixture across the stationary phase which is used to separate the compounds. Although in HPLC mobile phase is tailor made to suit the polarity of the analytes. The mobile phase used in this particular experiment was Methanol and 0.1M sodium dihydrogen phosphate at a ratio of 30:70 and a pH of 4.5, slightly acidic.
The reaction was stopped by adding 2 μL of 0.2 M EDTA at pH 8. Probe RNA was precipitated by centrifuging at 14,000 rpm at 4 degree Celsius for thirty minutes. The RNA transcripts were then analyzed by spectrometric and gel electrophoresis techniques. Spectrometric results showed an absorbance of 0.371 A at a wave length of 257 nm. We then calculated the concentration of RNA as 14.84 ng/μL, which
Halides Lab: Background information: Halide ions are reactive and useful. Salts are positively charged ions (metals) combined with any negative ions (nonmetal), and when placed in a solution (water) it separates into the cations and anions that made it up. The Purpose of this lab is to find out how the Halides react with the indicators, and to determine the identity of the two unknown solutions (A and B). Color of solutions prior to experiment: NaF | NaCI | KBr | KI | Unknown A | Unknown B | clear | clear | clear | clear | clear | clear | Color of indicator prior to experiment: 5% Bleach (NaOCI) | 0.2 M Na2S2O3 | 0.1 M AgNO3 | 0.5 M Ca(NO3)2 | clear | clear | clear | clear | Halide solutions | NaF | NaCI | KBr | KI | unknown A | unknown B | Test 1: Ca(NO3)2 | Cloudy White (Nothing) | Clear | Nothing | light yellow (Nothing) | Nothing | Nothing | Test 2, Part A: AgNO3 | clear (Nothing) | Milky White | Gold (Cloudy yellow) | milky green (Cloudy yellow) | turned white, film developed on top layer | Milky | Test 2, Part B: add Na2S2O3 to test tube from part A | Dark Orange/brown | Clear | Dark Gold(precipitate yellow then clear) | milky green (no change) | white precipitation, settled on bottom | Milky | Test 3: NaOCI (Bleach) | Clear (Nothing) | Nothing | Nothing | Orange (Clear) | Nothing | Orange | Unknown A is identified as NaCI (Sodium Chloride), because in test#1 the solution turned a cloudy white color when Ca(NO3)2 (Calcium nitrate) was added. In the first part of test#2, when AgNO3 (Silver nitrate) is added, the solution turned white, with a thin layer of film developing on the surface.
9) The number of moles of H2CO3 in the reaction NaHCO3 + C2H4O2 NaC2H3O2 + H2CO3 is also the number of moles of NaHCO3. 10) Calculate the number of grams of sodium bicarbonate by multiplying the number of moles of sodium bicarbonate by its molar mass. OBSERVATIONS BEFORE REACTION Total Mass: 165.93 g Total Mass: 165.93 g Mass: Alka Seltzer Tablet: 3.82 g Beaker + Vinegar: 162.11 g Temperature: 20.9 °C DURING REACTION Observations: Alka Seltzer is fizzing and dissolving into the vinegar Gas is being released as bubbles form; it’s carbon dioxide Looks like a nice glass of frothy beer AFTER REACTION Observations:
White precipitate shows the presence of chloride (Cl-). Chloride anion equation: HCl(aq) + AgNO3 (aq) → HNO3 (aq) + AgCl(s). The nitrate anion test involves cooling a mixture containing 1 mL of test solution and 3mL 18M H2SO4. 2mL is poured down the inner test tube side and the presence of a brown ring shows nitrate (NO3-) to be present. The carbonate anion test mixes 1 mL of test solution and drops of 6M HCl.