a) How do (1) temperature and (2) pH affect the activity of this enzyme? In your answer, include a discussion of the relationship between the structure and the function of this enzyme, as well as a discussion of how structure and function of enzymes are affected by temperature and pH.
b) Describe a controlled experiment that could have produced the data shown for either temperature or pH. Be sure to state the hypothesis that was tested here.
STANDARDS: MAX = 12 pts
Part a. (maximum 6 points)
Optimum temperature and pH concept [must include both temp and pH]
Enzyme/Substrate Fit concept (function dependent on conformation complementary between enzyme and substrate)
Tertiary (and sometimes quarternary) structure determines function
Description of enzyme structure or function, e.g.
Elegant description of primary to tertiary or primary to quarternary levels of structure Increases rate of reaction
Protein folding/coiIing Increases proximity of reactants
Co-enzymes/co-factors Decreases activation energy of the catalyzed reaction
Zymogens Decreases time to reach equilibrium
Allosteric effectors Induced fit and/or orbital steering ("bond stress")
Denaturation concept [temp and/or pH] linked to decreased enzyme activity (e.g. "denaturation" in context or unfolding or change in 3D shape. not "enzyme breaks down")
How temperature affects conformation (increased temperature breaks specific bonds e.g. hydrogen, Van dcr Wuals, eljsrtllide bridges)
How pH affects conformation (change in H concentration causes a change in specific bond interactions, e.g. hydrogen: ionic, R-group interactions)
Kinetics (increased or decreased molecular movement ) linked to effect on enzyme activity due to increase or decrease in temperature up to the optimum.
Part b. (maximum 6 points)
Experimental design must be relevant to the data shown
What is measured...