Do not go into detail about the protocol. The purpose of the experiment is to use a colorimetric assay to determine the optimal pH and temperature of a phosphatase enzyme found in a crude protein extract isolated from alfalfa sprouts. After conclusion of the experiment, it has been found that the pH optimum of the phosphatase enzyme was 5 and the temperature optimum was 55 degrees Celsius. The phosphatase enzyme was classified as acidic. 5.
The absorption spectrum is measured using a spectrophotometer and the data is graphed in Excel. The peak of the line is used to find Vmax of Fe2+. Vmax is used to find the moles of Fe2+ and ligand. The unknown n is a ratio of moles ligand divided by moles Fe2+. Results and Discussion For the first part of the experiment (Part A), five different 100 mL volumetric flasks were each filled with 1,2,3,4 and 5 mL of iron (II) solution.
Skip questions B, C, D, E and F in the lab manual, and answer these instead: A. What is the average % acetic acid in your vinegar sample? Standard vinegar is 5% acetic acid.How does your result compare with the standard? G. A 5.0 mL sample of vinegar was titrated with 7.2 mL of 0.55 M NaOH(aq). If the density of the vinegar solution is 1.00 g/mL, what is the mass percent of acetic acid present?
II. Introduction Part 1: The Effects of Concentration on Enzyme Activity In this experiment, the effects of concentration on enzyme activity were determined by using a spectrophotometer to observe absorbance changes taking place in samples containing a pH 5 buffer, guaiacol, hydrogen peroxide, and different volumes of potato extract. Hypotheses Ho: The amount of enzyme added does not influence the rate of reaction. Ha: The amount of enzyme added does influence the rate of reaction. Part 2: The Effects of Temperature on Enzyme Activity In this experiment, the effects of temperature on peroxidase activity were determined by using a spectrophotometer to observe absorbance changes taking place in temperature treated samples containing potato extract, guaiacol, hydrogen peroxide, and pH 5 buffer.
The purpose of this experiment was to determine the effects of substrate concentration and pH on the initial rate of enzyme-controlled reaction. METHODS I conducted five experiments. In each experiment, I had five test tubes with constant pH levels of an enzyme solution. The pH levels over the 5 experiments were 3, 5, 7, 9, and 11. I added varying levels of substrate to the test tubes in each experiment.
Prelaboratory preparation: Read pages 1 – 30 and 45 – 54 of the lab text. You may disregard the microscale distillation procedures of TECH 0704. Answer in your laboratory notebook the Safety Quiz on pages 11 – 12 and the Prelab Questions 1, 2a, 2b, 3 -5, pp. 67 - 68. EXPERIMENT: TECH 0704, Distillation, macroscale technique only, simple and fractional: Substitute a mixture of 25 mL ethanol and 25 mL water for the toluene/cyclohexane mixture.
. Experiment #2: Determining Effect of pH on Lactase Activity will involve students testing a range of different pH solutions mixed with cow milk to see if there is a change in the lactase activity. Access student prior knowledge on what conditions most affects enzyme activity (pH and temperature). Discuss what neutral, acidic, and basic (or alkaline) pH values are and where they are found in the pH range (0-14). Review the effect of pH on enzyme function.
Calculate the equilibrium constant, Ke. [0.85] 4. If the equilibrium concentration of F2(g) is 1.50 mol/L and H2(g) is 2.5 mol/L, determine the concentration of HF(g) at equilibrium. [1.92 mol/L] F2 (g) + H2 (g) === 2 HF(g) Ke = 0.98 5. If 0.100 mol of hydrogen iodide is placed in a 1.0 L container and allowed to reach equilibrium, find the concentrations of all reactants and products at equilibrium.
Name: ___________________________________ Date: ______________ Practice Test #3 ____ 1. When a precipitation reaction occurs, the ions that do not form the precipitate A) evaporate B) are cations only C) form a second insoluble compound in the solution D) are left dissolved in the solution E) none of these 2. An aqueous solution of potassium chloride is mixed with an aqueous solution of sodium nitrate. The complete ionic equation contains which of the following species (when balanced in standard form)? A) B) C) D) E) ____ 3.
Test Six: Glucose assay – Whether an organism can respire or ferment glucose can be tested with this Glucose O/F medium assay. Test Seven: Oxidation Fermentation assay (O.F.) – “The original intention of this medium was to be able to differentiate between Gram-negative bacteria that can: - Ferment - Only catabolise glucose by respiration - Do not catabolise glucose at all” (Singh, 2009). Test Eight: Motility assay –This technique will also be used to determine whether the unknown bacterium is Gram-positive or