* Secondly you have to add five drops of phenolphthalein the test tube which you are using. * Then measure out 5 cm of milk using a syringe or a measuring cylinder, and then add this to the test tube. * After this measure out 7 cm of sodium carbonate solution using a different measuring cylinder or a syringe and then add this to the test tube, which will make the solution turn pink. * Next you will have to place a thermometer in the test tube, but you will have to be careful as the experiment could fall over. * Then you will have to put the test tube in a water bath and leave it until the contents reach the same temperature as the water bath.
Plan Aim: The aim of this experiment is to identify the optimum pH for which the enzyme, amylase, works best at. Hypothesis: I predict that enzyme activity will decrease if it steers away from 6-8 pH as this is the optimum pH range for which the enzyme amylase works best. Variables: Dependant Variables: • The time it takes the enzyme to react. Control Variables: • The amount of starch solution • The amount of enzyme solution • The amount of Iodine solution • The amount of water in ML • The time taken for the reaction. Independent Variables: • The range of pH, between 6-8 pH.
Testing the reaction rate of fermentation based on alterations in ph solutions. Emily Anderson Abstract The experiment was conducted to determine what effect different ph solutions would have on the reaction rate of yeast fermentation. It was hypothesized that, the more acidic a ph solution, the slower the reaction rate would be in fermentation process. The reaction rate was judged by the amount of co2 displacent formed in the top of the fermentation tubes every five minutes for twenty minutes intervols. Introduction When the oxygen supply runs short in heavy or prolonged exercise, muscles obtain most of their energy from anaerobic process called glycolysis.
Also from pH 2 – pH 6.8 the absorbance did increase, but from pH 8 – 10 the absorbance decreased. Introduction Enzymes are an essential part of life; without enzymes inside our system most reactions would happen so slowly we would not be able to sustain life. With enzymes in our system our cells can go through reactions quickly and efficiently. An example of an enzyme we will be using in our experiment is Lactase. Lactase is the enzyme responsible for breaking down lactose, which is commonly found in milk.
Optimum: The term optimum is the greatest degree or best result obtained under specific conditions; it also is the maximum rate of reaction which works best at an assured level of temperature. If the optimum temperature is too high the enzyme will be denatured and if the optimum temperature is low this means the enzymes will work slowly. The optimum temperature for the concentration on the starch I choose was 2% of starch added to the substrate as it was in the middle and worked the best comparison to the other results, also I had accurate results of the concentration in starch and the result was 0.034 for the rate of reaction increased. Justification: The results I have for 30C was 0.20 and for 40C is 0.05 so this proves that the higher the temperature the enzymes get denatured and that in-between these two temperatures the enzyme works best at. Evaluation: To make this experiment more accurate with reliable results I need to make sure I repeat the practical at least 3 times in order to get the average temperature and results.
The peroxide value (PV) test, which is one of the most common tests used to evaluate the extent of lipid oxidation, is based on measuring peroxides. Objective: To measure the PV or a number of food samples, and to evaluate the meaning of the results. Reagents: Acetic acid (glacial) Chloroform (CCl4) 15% Potassium iodide (KI) 0.01 N (0.01M) sodium thiosulfate (Na2S2O3) Starch indicator 0.5 % concentrated hydrochloric acid HCl 0.01 N (0.00167M) potassium dichromate K2Cr2O7 (fix.) Procedure Determination of the titre of the sodium thiosulfate solution Measure off 10 ml of 0.01N K2Cr2O7 solution to a 200 ml conical flask. Add 0.5 ml concentrated HCl and 1.0 ml 15% KI solution.
It is important to replenish the body as soon as possible because even before an athlete feels thirsty, the earlier signs of dehydration may have already set in (Bucci, L., 1993). Carbohydrates are the body’s most efficient energy source. Over fifty percent of the body’s calorie intake should come from carbs. Carbs are broken down in complex and simple sugars that are used in the body in different ways; if not used immediately the extras are stored in the liver. If too many carbs are being taken in by the body, they are then
Enzyme Activity Lab By: Cheyenne Sherrill Lab partners: Brandon, Sade, and Jason Due date: November 27th 2012 Mr. Clark D1 honors Procedure: All the metabolic activity and processes within the cell require enzymes to occur. This includes synthesis and decomposition reactions. Enzymes are specialized proteins (organic catalyst) that speed up the rates of reactions that would otherwise be to slow or not able to occur at all in the cell. 1) Sand and peroxide- a pinch of sand and 2ml of hydrogen peroxide in a standardized test tube. Measure the height of the reaction with a ruler in millimeters.
This is not the case. Increasing levels of highly fatty foods over a prolonged amount of time is indiscriminante of age, gender or fitness level. Higher levels of saturated fats will increase the amount of cholesterol produced within the liver. The liver is a major site of biochemical activity within the body. Its homeostatic function is to convert food into other forms and remove excess cholesterol from the blood; any excess cholesterol is excreted as bile into the hepatic vein leading to the heart, thus increasing the amount of cholesterol circulated around the blood stream.
Moreover, refining not only makes whole foods durable and portable, but also concentrates their energy and speed them up. Industrial revolution brought in tremendous changes in nutrients components. One of the most momentous changes was the introduction of sugar and increase in the percentage of calories coming from sugar and carbohydrates. Because of removing fibres that slow down the release of glucose into the bloodstream from refined food, it is easier to digest. Because of refined food many people became prone to chronic diseases as heart diseases, and cancer.