Determination of Vitamin C Content in Beverages

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Introduction Ascorbic acid, also known as vitamin C, is an essential antioxidant needed by the human body. An antioxidant is any substance that prevents or inhibits the oxidation of another substance. Humans and other vertebrates are unable to synthesize vitamin C because there is a lack of gulonolactone oxidase, the last enzyme needed for ascorbic acid synthesis. This causes an outlook for other sources of the vitamin such as juices and tablets. Vitamin C is important in the daily lives of humans because it is required for the production of collagen, which forms scar tissue that heals wounds, mends fractures, and supports capillaries that resists bruises. Not only is vitamin C crucial for these functions to perform, it is also highly important for the production of thyroxine which is the hormone that regulates metabolic rate and body temperature. The downside of having to retrieve other sources of Vitamin C is deficiency and toxicity. When the intake of ascorbic acid is less than 10 mg a day, it can result in scurvy, a deficiency in vitamin C that generally affects those with poor diets and disease such as diabetes. On the other side of the spectrum, if ingestion of vitamin C is more than 10 grams, it can be a factor in the result of kidney stones, due to its toxicity. During titration, iodine is added and the ascorbic acid is oxidized. Once the ascorbic acid is oxidized, the excess iodine is free to react with the starch indicator. The endpoint of the titration is considered when the complexion of the titration is a blue-black color, meaning that the vitamin C is essentially gone. The purpose of this experiment was to determine the amount of vitamin C in various beverages using the regression equation from the standard curve. I proposed that the beverage that would contain the best source of vitamin C would be orange juice. Materials and Methods

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