Chem 131A: pH and Buffers Experiment This experiment is designed to review your background in pH and buffers and to expand upon it. The first section involves the titration of an unknown amino acid through its two equivalence points. The equivalence point data will allow you to determine apparent pK values which you will correct for ionic strength and the molar mass of your unknown. Further, you will examine how the amounts of the major species change along the titration. The second section examines the effect of ionic strength on the pK of a weak acid.
Name: Marc Dickson Course: Biology 100 Date: 2/7/2014 Lab Title: (Enzyme Controlled Reactions) Question: How do substrate concentration and pH affect enzyme controlled reactions? Introduction: Part of the enzyme is called the active site...The substrate binds to this part of the enzyme. The active site is shaped in a certain way that is coded to fit with its substrate. If the substrate has the right shape to attach itself to the enzyme's active site, it will. I will investigate and determine the effects of substrate concentration and pH on the initial rate of an enzyme-catalyzed reaction.
Lipids- The materials used in this section were Water, piece of brown paper bag, and vegetable oil. Procedures- Protein-In this experiment we are testing for protein in each solution. We pour drops of biuret reagent into the different contents. If the liquid turns purple, then protein is active. Carbohydrates- In this experiment We test for starch.
Each of the tubes were filled with various amounts depending on the table. Conclusion/Discussion The more BSA was added into each tube, the darker the solution was. The Biuret reagent, reducd the absorbance of light and proved my hypothesis correct: the more proteins in the solution, the darker the solution will be. The Biuret solution reacted with each tube resulting in different colors in every tube. Because this experiment was done several times, each one differently, one could tell by the graph how much unknown BSA was in the solution.
how are cells and bodies made from the instructions in DNA Regents Biology! Regents Biology! DNA ! Proteins ! Cells !
codon 3. What is the three letter base sequence on tRNA called? anticodon 4. What amino acid do proteins start with? (start codon) methionine 5.
Include in your chart, location, complementary base pairs, sugar, helix or single 19. What is the overall scheme to protein synthesis? 20. Describe the process of transcription? What is a code?
The structure of these proteins is decided by the side R-group found on the combination of amino acids. Primary structure consists of a polypeptide chain of amino acids. To make a
Honors Biology Chapter 4 Study Guide 1. Describe the structure of the organelles described in chapter 4. ex)nucleus has a double membrane and nuclear pores. very basic 2. If a cell has an extensive Golgi apparatus what function/process would you expect that cell to do? stores proteins and alot of exporting (sending stuff out of the cell) 3.
Procedure: Before beginning this lab please take the time to review this hand out and compare it to the information you know about organic molecules. Then, develop a hypothesis using the If, and Then statement. Go to http://www.okc.cc.ok.us/biologylabs/ and click on the “Organic Molecules” link. Read the introduction, and then proceed with tests for carbohydrates, proteins, fats, and foods. Record and answer all questions AS YOU GO.