Cell and Haemocytometer

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Experiment: Yeast Cells and Haemocytometer Research Question: What is the number of yeast cells per cm³ in different dilutions factor of yeast suspension? Hypothesis: The higher the dilutions factor of yeast suspension, the lesser the number of yeast cells. When the dilution factor increases means that the volume of the distilled water is higher than the number of yeast cells suspended in the solution. So the number of yeast cells will decreases. Variables: | | Units | Range | Independent variable | Dilution factor of yeast suspension | | 2-4 | Dependent variable | The number of yeast cells in one primary square | | | Controlled variables | Units | Method for control/possible effects on results | (1) Volume of distilled water | cm³ | 9ml of distilled water is used to diluted the yeast suspension | (2) Volume of yeast suspension | cm³ | 1ml of yeast suspension is taken from each of the test tubes during the dilution series | (3) Magnification of objective lens | | 10× of objective lens is used to focus on the primary square. Since different power of lenses have different field of view. | (4) Temperature | ̊ C | The experiment is carried out in the laboratory of room temperature of 28 ̊ C. | (5) Pressure | atm | The experiment is carried out in the laboratory under 1 atmospheric pressure. | Materials and Apparatus: Refer to handbook from page 163-167 Methodology: 1. The haemocytometer is cleaned with alcohol and wiped with tissues. 2. The slide is moistened using damp tissues. 3. The special coverslip is then pushed on to the slide and pressed down on the edges of the coverslip at the same time. 4. Then the yeast suspension is shaken gently. 5. A
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