from the penny. * Dry the penny and retest x4 * Determine the number of drops of Chocolate milk it takes to fill a penny, holding the eye dropped 2 cm. from the penny. * Dry the penny and retest x4. * Calculate data.
Brand name of the FD&C color(s) from the grocery store food = Blue #1, Red #40 C. The FD&C color(s) making up the grocery store food colorings = McKormicks- Yellow #42, Blue #1, Red #40 D. The FD&C color(s) making up the Kool-Aid drinks. = Grape= Red #3 and Blue #1, Strawberry= Red #3 E. The FD&C color(s) making up the M&M’s®. = Blue #1, Yellow #6, Red #3, Post-Lab Questions: 1.Why can't a pen be used to draw the baseline on the chromatography paper? Because the ink would separate. 2.
Radu Purtuc | Chemistry 231 - Lab | Section 32 Ronnie :: team-member TECH 705, SEPARATING ACIDS AND NEUTRAL COMPOUNDS BY SOLVENT EXTRACTION - MACROSCALE EXTRACTION July 6, 2006 Lab Report INTRODUCTION Purpose: using extraction technique to separate acetanilide, p-toluic acid, and p-tert-butylphenol; then measure the melting points of each compound and comparing them to the statistical values. Background: separating acids and neutral compounds by solvent extraction is a useful technique used in practice by chemists to separate organic compounds from mixture of other compounds. It generally involve several steps, with limiting factors being the number and output purity of compounds to be extracted from source mixture. A number of specific compound solvents not inter-miscible are used to separate and dissolve compounds, and a physical transfer between solvents is employed. An example of use is cleaning compounds of impurities, where different solvents work as a molecular filter that interacts differentially with compound and impurities respectively.
. Experiment #2: Determining Effect of pH on Lactase Activity will involve students testing a range of different pH solutions mixed with cow milk to see if there is a change in the lactase activity. Access student prior knowledge on what conditions most affects enzyme activity (pH and temperature). Discuss what neutral, acidic, and basic (or alkaline) pH values are and where they are found in the pH range (0-14). Review the effect of pH on enzyme function.
3. Plasmid pBR607 is a 2.6 kb plasmid containing Ampicillin and Tetracycline resistance markers, an origin of replication, and unique restriction sites for the restriction enzymes EcoRI, BamHI, and PstI. Given the restriction map for pBR607 for the enzymes EcoRI, BamHI, and PstI, show on the agarose gel picture below where the approximate positions of the restriction fragments generated from the given restriction digests would be located after carrying out electrophoresis. 4. As part of an undergraduate project, a student was attempting to construct a restriction map for the plasmid pUC23 using the restriction enzymes EcoRI and BamHI.
We found the mass of our pure alcohol to be 10.23g. The volume of our pure alcohol was 10.97mL. With these numbers, we used the formula mass/volume to find the density of our pure alcohol to be .9325g/mL. The percent error of our experiment was found to be 18.19% with an absolute error of .1435%. Conclusion:The purpose of this lab is to prepare and purify a fuel, ethanol C2H5OH.
Partner: Tyler Smith Data Table #1 Pure Solvent Mass of test tube + cork 45.502 g Mass of test tube, cork, + p-xylene 70.199 g Mass of p-xylene 24.697 g Data Table #2 Unknown Molecular Weight Determination Unknown letter C Mass of solid unknown 2.256 g Data Table #3 Results Freezing point of P-xylene, T°f 13.4 °C Freezing point of solution, Tf 10.8 °C Value for ΔTf 2.60°C Molality of unknown solution, m 0.60 m Mass of p-xylene, g 24.697 g Mass of p-xylene, kg 0.0247 kg Mols unknown solution 0.015 mols Mass of unknown added 2.256 g Molecular weight of unknown solute 1.5 x 10 2 g/mols 3. Show the calculation for obtaining the mass of p-xylene used in the experiment. (Mass of test tube, cork, + p-xylene ) - (Mass of test tube + cork) 70.199 g - 45.502 g = 24.697 g 4. Show the calculation for obtaining the mass of unknown added.
BIOSYNTHESIS OF ETHANOL Organic Chemistry 3441 March, 8, 2014 Abstract: The purpose of this experiment was to distil an ethanol-water mixture with simple and fractional distillation then determine the percentage of each compound in the mixture using a gas chromatograph with a thermal conductivity detector (GC-TCD). A mixture of disodium hydrogen phosphate, yeast, water, and sucrose was allowed to ferment with a calcium hydroxide trap and distilled with simple distillation yielding 57.7% ethanol and the fractional distillation resulted in 85.6%, in contrast to the original un-distilled sample that contained 10.7%. Had the distillations been performed at a slower pace, it is highly likely that higher ethanol concentrations could have been obtained. The evolution of carbon dioxide will drive out the residual air that is in the apparatus, and the test tube bubbler will ensure that no oxygen will diffuse back into the fermentation flask. Keywords: Ethanol, Simple Distillation, Fractional Distillation, Biosynthesis Introduction: It is well known that all throughout history, people have utilized yeast’s ability to convert sugars into ethanol primarily in hopes of an intoxicating beverage.
The yeast suspensions were not able to produce carbon dioxide from the control, lactose solution, or galactose solution (Table I). The greatest rate of carbon dioxide release for the central third of the elapsed time (from the 15 minute mark to the 30 minute mark) was 0.0313 mL/min for fructose solution followed by 0.0213 mL/min for sucrose solution, 0.206 mL/min for glucose solution, and 0.0200 mL/min for the maltose solution (Table II). The structure of each monosaccharide and disaccharide determines whether the enzymes present in the yeast can metabolize the sugar and produce carbon
The purpose of the laboratory is to determine the number of waters of hydration in aluminum potassium sulfate as well as the purity of the Alum we produce. Experimental1: The procedure for this laboratory was followed closely from Professor Abrams’ lab manual. In Part 1 of this laboratory an aluminum soda can was cut apart and the paint and lacquer was rubbed off with steel wool. Next, the aluminum can was rinsed off with tap H2O and then cut into small pieces. Using the analytical balance 1.0537 g of the aluminum can was weighed out.