Bio Lab Microscope Report

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The Microscope Microscope is a tool used to enlarge images of small objects that are hard to study with bare eyes. The compound light microscope, which is going to be used in this lab activity, is an instrument with two lenses and various knobs to focus the image. In this lab, we will learn about the proper use and handling of the microscope. Materials * Compound Microscope * Glass slide with the letter “e” on it Results As I viewed the letter, it was orientated upside down. You can’t really see the slide when you move it left or right or toward or away from me. It’s out of your field of view when you move it. When you go from 4x to 10x the brightness of the view dims. When the image is viewed through the high-power objective, the “e” is magnified 400x larger. If you didn’t know what you was looking at and was viewing the image from the high power objective, you wouldn’t know what it was because the higher the magnification the more obscure the slide becomes. Total Magnification and Areas of Field of View for Three Objectives Objective Power | Objective Magnification | | Ocular Magnification | | Total Magnification | 4x | 4x | x | 10x | = | 40x | 10x | 10x | x | 10x | = | 100x | 40x | 40x | x | 10x | = | 400x | Conclusion When handling a light microscope properly, there are two procedures that should be taken carefully. First, we should start observing an object from the low-power objective. To get the image focused when using the low-power objective, we should use coarse adjustment knob to adjust it first, not fine adjustment knob. Fine adjustment knob should be used later, such as when looking at high-power objectives. Otherwise, the slide and the objective can be damaged. Also, we should always treat the microscope with great care. To be specific, the microscope should be carried with two hands; one holding the arm, and another

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