What kinds of clinical specimens may yield a mixed flora in bacterial cultures? Oral, Skin, or GI specimens 5. When more than one colony type appears in pure culture, what are the most likely sources of extraneous contamination? Individual colonies can be picked up on the inoculating loop, or straight wire and inoculated in to the fresh agar or brother media References Cowan, M. K. (2012). MICROBIOLOGY: A SYSTEMS APPROACH, THIRD EDITION.
Review Sheet Week 8 Exercise 21.5 Questions 1. Morphology and culturing. Pneumococci are Gram-positive, oval to lancet- shaped cocci that usually occur in pairs or short chains Morphology refers to an organism's structure, form and appearance. Gram stains allow for better viewing of bacterial cell structure 2. Alpha hemolysis 3.
September17.2013 P.4 Environmental Sources Of Bacteria Purpose: We did this lab to find out exactly which conditions would help to grow a bacterium the best. Materials: • Agar • Petri dish • Cotton • Incubator • Tape • Bacteria Procedure: 1. Mark bottom of Petri dish 2. Split petri dish into 4 sections and label the sections 3. Pour hot agar into dish 4.
1.1 Identify the differences between bacteria, viruses, fungi and parasites. Bacteria: A bacteria is a single celled organism, they are either a parasite or live independently. Bacteria have three basic shapes. Antibiotics can usually kill them; A few examples of bacteria could be meningitis, Ear infections, and conjunctivitis. Fungi :A Fungus is a spore producing organism and can live as a single celled yeast or as a larger multi-cellular mould.
The streak plate technique is done to isolate a colony formed by a single cell from a mixture containing millions of cells. The streak plate technique was also used to obtain pure cultures of the bacteria using a trypticase soy agar (TSA) plate and was incubated at 37 ̊ for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the arrangement of the cells; if an organism appears purple under a microscope then it is said to be Gram positive, and if it
placed in a spectrophotometer. The data collected was used to view the growth of the bacteria and can be used to compare the growth of this E. coli strain to other E. coli strain, bacteria and the general bacterial growth curve. The data collected can make a template to possibly classify unknown bacteria. Not all bacteria replicate and grow at the same rate. Processes such as gram staining can be used in conjunction with monitoring the growth of a bacteria to further reinforce a hypothesis.
What is the frequency of tetracycline resistant bacteria in the cattle farm cultures? We will answer the questions by finding out if each bacteria is unique to a certain farm or all they all similar. Methods We performed serial dilutions on tetracycline plates. This helped us determine the frequency of tetracycline resistant bacteria I the original bacterial population. We used Polymerase Chain Reaction to amplify samples of the bacterial plasmid DNA.
The visual analysis of the results of the experiment will also be provided in which my qualitative data will be presenting which includes my own evaluation and observations of the experimental results. Quantitative & Qualitative Analysis The tables below show the results obtained by the first experiment in which I tested the effectiveness of TCP on E.coli Bacteria in 2 different petri
Ehrlichiosis Waukesha County Technical College Ehrlichiosis Ehrlichiosis is a bacterial disease caused by a tick bite. It can infect humans and animals and lives in the white blood cells of its host. This paper will be about the pathogens/virulence factors, pathogenesis, signs and symptoms, diagnosis, treatment, prevention, and epidemiology of ehrlichiosis. To start with, the pathogens of ehrlichiosis are three different types of bacterial species: Ehrlichia chaffeensis, Ehrlichia ewingii, and Ehrlichia muris-like (Centers for Disease Control and Prevention, 2011). The family the bacteria belong to is called Rickettsiae.
Flagellum is a crucial characteristic to the motility and colonization of Proteus mirabilis.it is also linked to the ability of this bacteria to form biofilms, assisting in the bacteria’s resistance to the host defenses and selecting antibiotics .It also acts as a surface sensor. P. mirabilis is a peritrichious flagellated bacterium .which gave it the ability for swarming motility. ( Armbruster and Mobely.2012; Belas and Suvanasuthi.2005). (32,124) These flagella form a bundle that propels the cells forward on the surrounding fluid(Tuson et al.,2013)(128) Proteus mirabilis elevates its flagellar number, from fewer than (10 to up to 5,000), and its length from (1 to 2 m )to (20 to 80 m), over differentiation from the swimmer to the swarmer cell type (McCarter,2010)(126) Adhesins fimbriae 1.4.1.2- Fimbriae are appendages on the bacterial surface which is used for adherence (Morgenstein.2011) (129), consisting of a polymer of a single polypeptide subunit interspersed with adhesive proteins. The fimbriae are arranged around the bacterial cell in a peritrichous form (Rocha et al.,2007)(130).