After all the acid has been completely added and the reaction has stopped, use a pipette to add a few extra drops of acid into the beaker until the reaction stops. 7. Place the beaker on a hot plate and heat on a high setting to boil off the water. WARNING: THERE WILL BE A SMALL AMOUNT OF HYDROCHLORIC ACID BOILING OF THE MIXTURE. DO NOT PLACE YOUR FACE DIRECTLY ABOVE THE BEAKER.
Discussion & Conclusion In this experiment we learned how to synthesize the cyclohexene by dehydration of cyclohexanol. We procedure the first step where we have to mix the components. Then we heat the R.B.F with a fractionating column, distilled water. Then we obtained the layers, and we transferred the organic layer to a small, dry Erlenmeyer flask. We added anhydrous Sodium Sulfate as a drying agent.
8. Use tongs to move the beaker from the hot plate to the lab bench. Wait until the beaker cools down. 9. Filter the solution with a funnel and a piece of filter paper.
Then after agitating the seaweed to remove monosodium glutamate and pouring off the water, 40 mL of distilled water was added to the seaweed and heated. During this procedure, the iodine extract was filtered and several tests, both standard procedure and home- Objective: The purpose of this experiment is to understand how a single substance can be isolated from a mixture, like seaweed and water. Also, learn how to utilize filtration and extraction to make a tincture for testing for Iodine, Iodide Ion, and Triiodide Ion. Procedure: 1. 6 grams of dried seaweed, cut into ½ inches should be places into a 150 mL beakers that will later be filled with ¾ distilled or deionized water.
Allow the splint to burn until the color fades. Don’t let any solids to fall into the burner. -Soak the wooden splint in the “rinse water” to put it out completely; move it to trash. -Record observations on flame color produced by the metallic salt in the Data Table. -Repeat above steps (middle section) for all metallic salts (five total).
Obtain an Erlenmeyer flask that has a vacuum opening and attach the vacuum tube to it 8. Insert funnel and rinse the funnel with distilled water 9. Turn vacuum on and pour the pink solution into the Erlenmeyer flask, the precipitate should stay on top of the filter 10. Rinse the beaker out until all residue is gone 11. Try to rinse the precipitate in the funnel until the pink color is gone 12.
| | | | | | |Washing a contaminated scope. (Gastro |Once a contaminated scope has been used and then come through into the dirty side off scope | |scope/Colon scope) |wash, I first fill my sinks with water and put one squirt off disinfectant which is Thermoton| | |into the dirty side off the sinks. | | |I then put on my PPE which includes | | |Red apron
The beaker was then moved to a stir plate with a stir bar and held in place by a ring stand. A funnel attached to an aspirator hose was fixed above to serve as a fume hood. With the aspirator and stir plate both on, 4.79 mL of 6 M HNO3 was added slowly to the beaker until the copper dissolved and the solution turned a light blue color. The hood could now be removed and 10.02 mL of distilled water was added to the solution. While stirring, 6 M NaOH was added drop-wise until the solution became basic, turning red litmus paper blue.
Add a drop of water using and inoculating loop to a clean glass slide. Then using a sterilized loop, smear the slide with the organism, let the slide dry completely and heat fix. Apply crystal violet stain (primary stain) for one minute, briefly rinse with distilled water. Apply iodine (mordant) for one 1 minute, briefly rinse with distilled water. Then proceed to decolorize with 95% ethanol until the ethanol running off the slide is clear.
Pour hot agar into dish 4. Keep lid off and let agar steam 5. Put top back on and let plate cool 6. Collect piece of bacteria by dabbing it with moistened cotton 7. Dab contaminated cotton onto section of the dish 8.